LOCUS       sequence-191417-        3842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing HOXB3 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-HOXB3
FEATURES             Location/Qualifiers
     source          1..3842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1297..1396)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1413..1432)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1414..1432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1437..1453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1437..1453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1555..1574)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1566..2375
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1633..1652)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2522..3110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3011..3030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3264..3281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3440..3467)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3559..3645)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3694..3716
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3708..3725
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3709..3725
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3741..3840
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgcagaaag ccacctacta cgacaacgcc gccgctgccc tgttcggagg ctacagcagc
       61 taccccggca gcaacggctt cggcttcgac gtgcctcctc agcctccctt ccaggccgct
      121 acacacctgg agggcgacta ccagagaagc gcttgcagcc tgcagagcct gggcaacgct
      181 gccccccacg ccaagtctaa ggagctgaat ggatcttgca tgaggcccgg cctggcccct
      241 gagcctctga gcgcccctcc cggctcaccc cctccttctg ccgctcctac atctgctaca
      301 agcaactctt ctaacggcgg cggcccctcc aagagcggcc cccccaagtg cggccccggc
      361 accaacagca ccctgaccaa gcagatcttc ccctggatga aggagagcag acagaccagc
      421 aagctgaaga acaactctcc cggaacagct gaaggctgtg gagggggagg tggaggaggc
      481 ggaggaggag gttcaggcgg atctggaggc ggcggtggtg gaggtggcgg tggagataaa
      541 agcccacctg gaagcgccgc cagcaagaga gccagaaccg cctacaccag cgcccagctg
      601 gtggagctgg agaaggagtt ccacttcaac agatacctgt gcagacccag aagagtggag
      661 atggccaacc tgctgaacct gagcgagaga cagatcaaga tctggttcca gaacagaaga
      721 atgaagtaca agaaggacca gaaggccaag ggcctggcta gcagcagcgg aggccctagc
      781 cccgccggca gccctcccca gcctatgcag agcacagccg gcttcatgaa tgcccttcac
      841 agcatgaccc ccagctacga gagcccctct ccccccgcct ttggcaaggc tcaccagaac
      901 gcctacgccc tgcccagcaa ctaccagccc cctctgaagg gctgtggcgc cccacagaag
      961 taccctccta cccccgcccc cgagtacgaa ccccacgtgc tgcaggccaa tggaggcgcc
     1021 tatggaaccc ctacaatgca gggctctcct gtgtacgtgg gagggggcgg ctacgctgat
     1081 cccctgcccc cacccgccgg acccagcctg tacggactga atcacctgtc ccaccatccc
     1141 agcggcaatc tggactacaa cggcgctcct cctatggctc ccagccagca ccacggcccc
     1201 tgtgagcccc accctaccta cacagatctg agcagccacc atgctcctcc cccccagggc
     1261 agaatccagg aggcccccaa gctgacccac ctgtataccc agctttcttg tacaaagttg
     1321 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     1381 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     1441 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     1501 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     1561 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     1621 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     1681 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     1741 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     1801 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     1861 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1921 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1981 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     2041 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2101 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     2161 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2221 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2281 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2341 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     2401 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
     2461 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2521 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2581 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2641 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2701 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2761 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2821 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2881 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2941 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3001 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3061 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3121 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3181 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3241 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3301 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3361 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3421 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3481 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3541 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3601 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3661 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg
     3721 ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt
     3781 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct
     3841 ta
//
