LOCUS       sequence-19143-e         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf60
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             339..380
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             390..407
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(430..447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    436..547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 accgaattcc agctgaccac catgaagtac gatgttattg tttactggta tatctggaaa
       61 gaaatctacc agtctaggaa taagagattg atatcgcata gaaatagtaa agtttataat
      121 ctcatcatcg aagagcattt tgttaccatt gtaataaata tccactctgt catatgtata
      181 aatgaagtac tgttcaaaca tgatgagatg tttatatgtt ggcatagtag tgagatcgac
      241 gtttggtaag caattcccgg ggatccaagg gtcaagacaa ttctgcagat atccagcaca
      301 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
      361 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
      421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
      481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      541 ttgcatcgca ttgtctgagt aggtgtcatt c
//
