LOCUS       sequence-191435-        3836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF275 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF275
FEATURES             Location/Qualifiers
     source          1..3836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1291..1390)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1407..1426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1408..1426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1431..1447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1431..1447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1549..1568)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1560..2369
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1627..1646)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2516..3104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3005..3024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3258..3275
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3434..3461)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3553..3639)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3688..3710
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3702..3719
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3703..3719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3735..3834
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgatgagcc acccttgcgt gagcctgctg ggagtgcccg tgctgaatcc tgcactggtg
       61 ccccacttgg cccagggcca ggtcctgctc gtgtctgatc ctagccccaa caccgaccct
      121 gccaagtaca gcgagagcac ctccgccacc agacaccaga tgaagggcga ggacgcccag
      181 ccccaggaaa tggccagcac aagcttccct agagctagcg gccccagccc agagttcaga
      241 cagcacggcg acagcgacgg caagagaggc agcccccaga acctgcccat cgagcaccac
      301 ttcgcctgca aggagtgcgg cgataccttc agactgaagg tgctgctggt gcagcaccag
      361 agagtgcaca gcgaggagaa gggctgggaa tgcggcgact gcggcaaggt gttccggggc
      421 gtggccgagt tcaatgagca tagaaagtcc cacgtggccg ccgagcctca gccaggccct
      481 agcagagccc tggagaacgc tgccgagaag agagagcaga tggagagaga ggccaagccc
      541 ttcgagtgtg aggagtgtgg caaaagattc aagaagaacg ccggactgag ccagcacctg
      601 agggtgcact ccagagagaa gccctttgac tgcgaagaat gcggaagaag cttcaaggtg
      661 aacacccacc tgttcaggca ccagaagctg cacacctccg agaagccttt tgcttgcaag
      721 gcctgcagca gagacttcct ggacagacag gagctgctga aacaccagag gatgcacacc
      781 ggccatctgc ctttcgactg cgacgactgt ggaaagagct tcaggggcgt gaacggcctg
      841 gccgaacacc agcggatcca cagcggcgcc aagccttacg gctgccccca ctgtggcaag
      901 ctgttcagaa gatccagcga gctgaccaag caccgcagaa tccataccgg cgaaaagcca
      961 tacgcctgcg gacagtgcgg caaagccttc aggcagtcca gctccctgct ggagcacgcc
     1021 aggatccact ctggcgagag gccttatgcc tgcggcgagt gcggaaaggc tttcagaggc
     1081 ccctccgacc tgatcaagca cagaagaatt cacagtggcc tgaagcccta cgagtgcgac
     1141 aagtgcggga aggcctttag gagaagcagc ggcctgagca gacacaggag aatccactcc
     1201 ggcgccagaa gatgcgagtg cagccagtgt ggccgggtgt ttaagagaag aagcgccctg
     1261 cagaagcacc agcccaccca ccacgagtat acccagcttt cttgtacaaa gttggcatta
     1321 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1381 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1441 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1501 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     1561 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
     1621 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     1681 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     1741 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     1801 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     1861 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     1921 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     1981 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     2041 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     2101 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2161 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     2221 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2281 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2341 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     2401 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     2461 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2641 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3181 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3241 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3301 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3361 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     3421 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     3481 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     3541 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     3601 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     3661 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     3721 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     3781 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggctta
//
