LOCUS       sequence-191445-        3797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing FOXD4L5 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-FOXD4L5
FEATURES             Location/Qualifiers
     source          1..3797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1252..1351)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1368..1387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1369..1387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1392..1408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1392..1408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1510..1529)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1521..2330
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1588..1607)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2477..3065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2966..2985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3219..3236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3395..3422)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3514..3600)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3649..3671
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3663..3680
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3664..3680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3696..3795
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgaacctgc cccgggccga gaggcccagg agcacccccc agcgctctct gagagacagc
       61 gatggcgagg atggcaagat cgatgtgctg ggcgaggagg aggacgagga cgaagtggag
      121 gatgaggaag aggaggcccg gcagcagttt ctggaacagt ctctgcagcc tggcctccag
      181 gtggccagat ggggcggcgt ggccctgccc agagaacata tcgagggcgg cggcggacct
      241 agcgacccta gcgagtttgg cacaaagttc agagctcctc ccagaagcgc tgccgccagc
      301 gaggacgcca gacagcccgc caagcccccc tacagctaca tcgccctgat caccatggcc
      361 atcctgcaga acccccacaa gagactgacc ctgtccggca tctgcgcctt catcagcggc
      421 agattcccct actacagaag aaagttcccc gcctggcaga acagcatcag gcacaacctg
      481 tccctgaacg actgcttcgt gaagatcccc agagagcccg gccaccccgg caagggcaac
      541 tactggagcc tggaccccgc cagccaggac atgttcgaca acggcagctt cctgaggaga
      601 aggaagagat tcaagagaca ccagctgacc cccggagccc atctgccaca cccttttccc
      661 cttcctgccg ctcacgccgc tcttcacaat cctcaccctg gccctctgct gggagcccca
      721 gcccctcctc agcctgtgcc tggcgcctac cctaacacag cccctggaag atgcccttac
      781 gccctgctgc accctcaccc cctgagatac ctgctgctgt ccgccccagt gtacgccgga
      841 gctcctaaga aggccgaggg cgccgatctg gctacccccg cccctttccc ctgttgtagc
      901 ccccacctgg tgctgagcct gggcagaaga gctagagtgt ggagaagaca cagagaggcc
      961 gacgccagcc tgagcgccct gagagtgctg tgcaagggct ctggcgagag agtgcagggc
     1021 ctgagaagag tgtgccccag acccagaggc gccaccgcta cctgcagcag cgaccaccag
     1081 gcctgctgta tccccaaacc cctgcccctg tgctgcaagt gtcccccccc actgctgctc
     1141 ggccagttct gctccaacag ctctagcatc agaagaaccg ctcccaccgc tgccctcccc
     1201 cccagagcca gatgctgggc cggcacctgt agacccagga gaagatgcta tacccagctt
     1261 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     1321 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     1381 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     1441 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     1501 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     1561 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     1621 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     1681 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     1741 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     1801 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     1861 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1921 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1981 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     2041 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     2101 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     2161 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     2221 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     2281 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     2341 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     2401 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2461 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2521 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2581 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2641 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2701 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2761 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2821 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2881 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2941 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3001 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3061 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3121 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3181 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3241 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3301 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     3361 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3421 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3481 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3541 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3601 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     3661 gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga
     3721 tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt
     3781 acaaaaaagc aggctta
//
