LOCUS       sequence-191477-        4628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF441 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF441
FEATURES             Location/Qualifiers
     source          1..4628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2083..2182)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2199..2218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2200..2218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2223..2239)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2223..2239)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2341..2360)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2352..3161
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2419..2438)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3308..3896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3797..3816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4050..4067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4226..4253)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4345..4431)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4480..4502
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4494..4511
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4495..4511
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4527..4626
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggacagcg tggccttcga ggacgtggcc atcaacttta cctgcgagga gtgggccctc
       61 ctgggaccca gccagaagag cctgtacaga gacgtgatgc aggagacaat caggaacctg
      121 gactgcatcg gcatgatctg gcagaaccac gacatcgagg aggaccagta caaggacctg
      181 agaagaaacc tgagatgcca catggtggag agagcctgcg agatcaagga caacagccag
      241 tgcggcggcc cctttaccca gacacaggac agcatcgtga acgagaaaat ccccggcgtg
      301 gacccttggg agtccagcga atgcaccgac gtgctgatgg gcagaagcag cctgaactgc
      361 tacgtgagag tggactctga gcacaagccc tgcgagtacc aggagtacgg cgagaagcca
      421 tacactcaca ctcaatgcgg caccgccttt agctaccagc cctgcttcca gatccatgag
      481 aggccccagc acggcaagaa actgtacgac tgtaaggagt gtgccagctt cagctctctg
      541 gagaacctgc agcggcacat ggccgcccac cacggagacg gacctagaat ttgcaagctc
      601 tgcggaaacg ccttcatctg gccctccctg ttccacatgc tgagaaggac tcacaccgag
      661 gagaagcctt atgagtacga gcagtgctct accgccttcc ccgcctacag cagcaccctg
      721 cgacatgaga gaacccacag cggagaaaaa ccttaccagt gcaaacagtg cggtaaagcc
      781 ttctcttgca gctgctacac ccagctgtac gagcgcaccc acaccggaga gcagagctac
      841 gaatgtaagc agtgcgggaa agcattttac cacctgggct ccttccagag acatatgatc
      901 gtgcatacag gagacgggcc ccacaaatgt aagatctgcg ggaagggatt cctgtccccc
      961 agcagcgtga ggagacacaa gagaactcac acaggggaga aaccctacga gtgtaagtat
     1021 tgtggcaagg ccttcagcga ctgcaccgga ttcaggaggc acatgatcac acacacaggc
     1081 gacggccctc acaagtgtaa ggtgtgtggt aaagcattcg acagccccag cctgtgcaga
     1141 aggcatgaga caacacacac cggtgaaaag ccatataagt gcgaatgtgg aaaggctttc
     1201 tccgacttct actacttcag aaaccacgag acaacccata ccggtgagaa gccgtataaa
     1261 tgtaaacagt gtgggaaggc attcatctgc tgcacctacc tgcagattca cgagaggatc
     1321 cacaccggcg agcggcccta taagtgtaag caatgcggta aggcctttcg gagcagcaac
     1381 tacatcagag tgcacgagaa gacccacaca ggagagaaac cttatgaatg caaacaatgc
     1441 ggaaaagctc tgagccacct gaagagcttc cagcgccaca tgataatgca tactggcgac
     1501 ggaccccata agtgcaagat ctgtggcaag tccttcgata gccctagcag ctttagaaga
     1561 catgagcgga ttcacactgg cgagaggcct tataaatgca agctgtgcgg aaagggcttc
     1621 aggagcagct cctacatcca gctgcacgaa agaacacata ccggcgaaaa gccctatggc
     1681 tgccagcagt gtggcaaagc cctgagcgac ctgagctcct tcagaagaca catgattacg
     1741 cacaccggga acggccccca caagtgcaaa atctgcggca aggggttcga ctacccctcc
     1801 agcgtgcaga gacacgaaag gacacacacg ggcgagaaac catacgagtg caaggagtgc
     1861 ggcaaagctt tcagccacag cagctacctg cggatccacg agcgggtgca cactggagag
     1921 aagccctaca aatgcaaaga gtgtggaaaa ccctttcact gccccagcgc cttccacaag
     1981 cacgagagaa cacactccat ggaaaagcct tacaagtgta aagaatgcgg cgaggctttc
     2041 cactgcatca gctcctttca caaacacgag atgacccact atacccagct ttcttgtaca
     2101 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2161 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     2221 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2281 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2341 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     2401 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2461 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     2521 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2581 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2641 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2701 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2761 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     2821 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     2881 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     2941 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3001 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     3061 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     3121 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3181 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3241 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4141 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4201 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4261 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4321 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4381 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     4441 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     4501 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
     4561 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
     4621 caggctta
//
