LOCUS       sequence-191489-        4478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF799 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF799
FEATURES             Location/Qualifiers
     source          1..4478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1933..2032)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2049..2068)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2050..2068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2073..2089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2073..2089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2191..2210)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2202..3011
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2269..2288)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3158..3746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3647..3666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3900..3917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4076..4103)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4195..4281)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4330..4352
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4344..4361
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4345..4361
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4377..4476
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggccagcg tggcactgga ggacgtggcc gtgaacttca ccagagagga gtgggccctg
       61 ctgggccctt gccagaagaa cctgtacaag gacgtgatgc aggagacaat cagaaacctg
      121 gactgcgtgg gcatgaagtg gaaggaccag aacatcgagg accagtacag ataccccagg
      181 aaaaacctga gatgcagaat gctggagaga ttcgtggaga gcaaggacgg cacccagtgc
      241 ggagagacaa gcagccagat ccaggacagc atcgtgacca agaacaccct gcctggcgtg
      301 ggcccctacg aaagcagaat gagcggcgag gtgatcatgg gccacagctc cctgaattgt
      361 tacatcagag tgggcgccgg ccacaagcca tacgagtacc acgagtgcgg tgagaagcca
      421 gacacccaca agcagagggg caaggctttc tcctaccaca acagcctgca gacccacgag
      481 aggctgcaca ctggcaagaa accttacaac tgtaaggaat gtggcaagag cttcagcagc
      541 ctgggcaacc tgcagagaca tatggccgtg cagagaggcg acggccccta taaatgcaag
      601 ctgtgcggca aagccttttt ctggcccagc ctgctgcaca tgcatgagag aacacataca
      661 ggggagaaac catatgagtg taagcagtgt agcaaagctt tcagcttcta cagctcctac
      721 ctgagacacg agcgcaccca taccggagag aagctgtacg aatgcaagca gtgctctaaa
      781 gccttccccg actacagcag ctgtctgaga catgaaagaa cccacaccgg aaagaagcca
      841 tatacctgca agcaatgtgg taaagctttt agcgccagca ccagcctgag gaggcatgag
      901 actactcaca ccgacgaaaa gccctatgcc tgccagcagt gtggcaaagc attccaccac
      961 ctgggcagct tccagaggca catggtgatg cataccagag acggacccca caaatgcaaa
     1021 atctgtggga agggcttcga ctgcccctca agcctgaaga gccacgagcg gactcacaca
     1081 ggagagaaac tctacgaatg taagcaatgc gggaaggccc tgagccacag cagcagcttt
     1141 aggagacaca tgaccatgca caccggcgat ggcccccaca agtgtaagat ctgtggcaag
     1201 gcattcgtgt accccagcgt gttccagcgg cacgagaaaa cacacacagc cgagaaaccc
     1261 tataagtgca aacagtgtgg gaaagcctac agaatcagca gctctctgag aagacatgag
     1321 acaacccata caggagaaaa accctacaag tgcaagtgcg gtaaggcctt tatcgacttc
     1381 tactccttcc agaaccacaa gaccacccac gccggagaaa agccttacga gtgcaaggaa
     1441 tgcggaaaag cattcagctg cttccagtac ctgtcccagc acagaaggac ccacacaggc
     1501 gaaaaaccgt atgaatgtaa cacatgcaag aaggcattct cccacttcgg caatctgaag
     1561 gtgcacgaga gaatccactc cggcgagaag ccttatgaat gcaaggagtg cgggaaagca
     1621 tttagctggc tgacctgctt cctgaggcac gaaagaattc acatgaggga gaagccctac
     1681 gagtgtcagc agtgcggcaa ggccttcacc cactccagat tcctgcaggg ccacgagaag
     1741 acccatactg gcgagaaccc gtacgagtgt aaagagtgcg gaaaggcatt tgccagcctg
     1801 tctagcctgc acagacacaa aaagacacat tggaagaaga cacacaccgg ggagaacccc
     1861 tacggatgta aggagtgtgg aaaggccttt gcctccctga gcagcctcca caggcacaag
     1921 aagactcact atacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     1981 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     2041 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     2101 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2161 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2221 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2281 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2341 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2401 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2461 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     2521 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2581 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2641 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2701 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     2761 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2821 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2881 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     2941 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3001 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     3061 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3841 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3901 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3961 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4021 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4081 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4141 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4201 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4261 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     4321 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
     4381 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     4441 tttttataat gccaactttg tacaaaaaag caggctta
//
