LOCUS       sequence-191497-        3807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing FOXI3 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-FOXI3
FEATURES             Location/Qualifiers
     source          1..3807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1262..1361)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1378..1397)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1379..1397)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1402..1418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1402..1418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1520..1539)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1531..2340
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1598..1617)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2487..3075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2976..2995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3229..3246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3405..3432)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3524..3610)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3659..3681
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3673..3690
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3674..3690
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3706..3805
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggccctgt actgcggcga taatttcggc gtgtacagcc agcctggcct tccaccacca
       61 gctgctacag ccgctgctcc tggagcacca cccgctgcta gagctcctta tggattggct
      121 gattacgccg ctcctcctgc tgccgcggct aatccctacc tgtggctgaa cggacctgga
      181 gttggaggac ctccttcagc tgcagcagct gccgcagctg catatttagg tgctcctcca
      241 cctccccctc ctccaggtgc tgctgctggc ccttttttac agccccctcc agcagccggc
      301 acatttggct gttctcaaag accttttgcc caacctgccc ctgccgcacc agcttctcct
      361 gccgctccag ctggtcctgg agaactgggc tggctctcca tggcctctag agaggacctg
      421 atgaagatgg tgaggccccc ctacagctac agcgccctga tcgctatggc catccagagc
      481 gcccccgaga gaaagctgac cctgtcccac atctaccagt tcgtggccga cagcttcccc
      541 ttctaccaga gaagcaaggc cggctggcag aactccatca gacacaacct gtccctgaac
      601 gactgcttca agaaggtgcc cagagacgag gacgaccccg gcaagggcaa ctactggacc
      661 ctggacccca actgcgagaa gatgttcgac aacggcaact tcagaagaaa aagaaagaga
      721 agaagcgaag cctctaatgg ctctaccgtg gccgctggca ccagcaagtc agaggagggc
      781 ctgagctctg gcttaggcag cggcgtcgga ggaaagcctg aggaggagtc tccttctaca
      841 ctgctgaggc ctagccatag ccctgagcct cccgagggca ccaagagcac agcctcttct
      901 cccggcggac ccatgcttac aagcaccccc tgcctgaata ccttcttcag ctccctgtcc
      961 agcctgtctg tgagcagctc cgtgagcaca cagagagccc ttcccggctc tagacacctg
     1021 ggcattcagg gagcccagct gccttctagc ggcgtgttta gccccaccag catcagcgag
     1081 gcctctgctg acaccttgca gctgtctaac agcacaagca acagcaccgg ccagcgaagc
     1141 agctactact cccccttccc cgctagcacc agcggcggcc agagcagccc ttttagcagc
     1201 ccctttcaca atttcagcat ggtgaacagc ctgatctacc ccagagaggg cagcgaggtg
     1261 tacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1321 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1381 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1441 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1501 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1561 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1621 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1681 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1741 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1801 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1861 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1921 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     1981 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2041 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2101 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2161 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2221 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2281 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2341 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2401 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3241 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3301 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3361 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3421 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3481 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3541 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3601 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3661 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta
     3721 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
     3781 ccaactttgt acaaaaaagc aggctta
//
