LOCUS       sequence-19150-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf67
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             621..662
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             672..689
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(712..729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cttggtaccg agctcggatc cagtaccctt caccgaattc cagctgacca ccatgattat
       61 caagtcttat acgatccgcg tcttttttga caggatcagg ttcttctaca ggaagaagtt
      121 tcggcctctt atgatattca tgtctgggaa acggtggtct agggtgaggc tccggtatcg
      181 gagtgggttt tggattataa tcatcatcgt ctatgacatc atcttcgact tcgatattta
      241 ttttgctatc ttgatgatgt cctgtatcag ttgcattttc agcactcgac tgaatattag
      301 cgcattcatt gtctattatt accatatttc taaacccaaa atgtatgtgt tgaacatcag
      361 tactatcgtt gatgagtctt atagcatgaa ttcgcttatc gttatcgggt ttatcttctg
      421 tcaccttagc aattcctttt ttattaaact ctacataatc atatccattt ctattgtttg
      481 ttctaatata aacgagtata gcatcattgc taaatttttt caataggcaa ttcccgggga
      541 tccgaataaa gggtcaagac aattctgcag atatccagca cagtggcggc cgctcgagtc
      601 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      661 cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
      721 tgccttctag ttgccagcca tctgttgttt tgccctcccc gtgccttcct tgaccctgga
      781 aggtgcactc ccactg
//
