LOCUS       sequence-19152-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf69
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             509..550
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             560..577
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(600..617)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    606..717
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcggatccag tacccttcac cgaattccag ctgaccacca tggcatctat tttaaataca
       61 cttaggtttt tggaaaaaac atcattttat aattgtaacg attcaataac taaagaaaag
      121 attaagatta aacataaggg aatgtcattt gtattttata agccaaagca ttctaccgtt
      181 gttaaatact tgtctggagg aggtatatat catgatgatt tggttgtatt ggggaaggta
      241 acaattaata atctaaagat gatgctattt tacatggatt tatcatatca tggagtgaca
      301 agtagtggag caatttacaa attgggatcg tctatcgata gactttctct aaataggact
      361 attgttacaa aagttaataa ttatgatgat acattttttg acgacgatga ttgagcaatt
      421 cccggggatc cgaataaagg gtcaagacaa ttctgcagat atccagcaca gtggcggccg
      481 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
      541 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
      601 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      661 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgagggaa ttgcatcgca
      721 ttgtctgagt aggtgtcatt ctat
//
