LOCUS       sequence-19153-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf70
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             382..423
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             433..450
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(473..490)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    479..703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgaattcca gctgaccacc atgtttaact tttgttctat gacgtcctta tcagcggtat
       61 cggtacatat acgtaattca ccttcacaaa atacggagtc ttcgataata atagccaatc
      121 gattattgga tctagctgtc tgtatcatat tcaacatgtt taatatatcc tttcgtttcc
      181 cctttacagg catcgatcgt agcatatttt ccgcgtctga gatggaaatg ttaaaactac
      241 aaaaatgcgt aatgttagcc cgtcctaata ttggtacgtg tctataagca attcccgggg
      301 atccgaataa agggtcaaga caattctgca gatatccagc acagtggcgg ccgctcgagt
      361 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
      421 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
      481 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      541 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      601 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
      661 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
      721 accagctggg gctctagggg gta
//
