LOCUS       sequence-19154-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf71
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             674..715
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             725..742
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgaattcc agctgaccac catgatgaca ccagaaaacg acgaagagca gacatctgtg
       61 ttctccgcta ctgtttacgg agacaaaatt caaggaaaga ataaacgcaa acgcgtgatt
      121 ggtctatgta ttagaatatc tatggttatt tcactactat ctatgattac catgtccgcg
      181 tttctcatag tgcgcctaaa tcaatgcatg tctgctaacg aggctgctat tactgacgcc
      241 gctgttgccg ttgctgctgc atcatctact catagaaagg ttgcgtctag cactacacaa
      301 tatgatcaca aagaaagctg taatggttta tattaccagg gttcttgtta tatattacat
      361 tcagactacc agttattctc ggatgctaaa gcaaattgca ctgcggaatc atcaacacta
      421 cccaataaat ccgatgtctt gattacctgg ctcattgatt atgttgagga tacatgggga
      481 tctgatggta atccaattac aaaaactaca tccgattatc aagattctga tgtatcacaa
      541 gaagttagaa agtatttttg tgttaaaaca atgaactaag caattcccgg ggatccgaat
      601 aaagggtcaa gacaattctg cagatatcca gcacagtggc ggccgctcga gtctagangg
      661 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
      721 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgcct
//
