LOCUS       sequence-191555-        5636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF197 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF197
FEATURES             Location/Qualifiers
     source          1..5636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3091..3190)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3207..3226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3208..3226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3231..3247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3231..3247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3349..3368)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3360..4169
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3427..3446)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4316..4904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4805..4824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5058..5075
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5234..5261)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5353..5439)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5488..5510
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5502..5519
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5503..5519
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5535..5634
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgacaagag aaaatgttgc ccataatgct ttgagacaag aaggccttgt aaaaggaaaa
       61 gatgacacgt ggaagtgggg tacatctttc caaggaagtt cttcgagtgt atgggaaacc
      121 agccacctac atttcagaca attgagatac catgaaacga gtggcccaca agaagccctt
      181 tcaagattaa gagaattatg caggaggtgg ctaagaccag aggcaagaac aaaagcccaa
      241 atcctagaac tccttgtcct tgaacaattc ctttcaattt tacctggtga gatcagaacg
      301 tgggtccaat tacaccaccc tggatctggt gaagaagctg tagcccttgt agaagaactc
      361 caaaaagacc tagatgggcc ggcaatccaa gtgcctgtcc ttgtgaaaga ccaagacacc
      421 ctacaaaaag ttgtatctgc acctgggacc actttgccgc ctgttttgcc tggatcccat
      481 attgcagcag aaatctgccc ccacccacca actgaccttg tggcttttaa cctccaagac
      541 ccccaacatg attcccctgc ccctgaggca agtgccctca gccaagaaga aaaccccaga
      601 aaccaattaa tggctttaat gctcctaact gcccagcccc aagaacttgt aatgtttgaa
      661 gaagtaagtg tgtgttttac cagtgaagag tgggcgtgtt taggaccaat ccagagggcc
      721 ctatactggg atgtgatgct agaaaattat ggaaatgtta ccagcctaga gtgggaaaca
      781 atgacagaaa atgaagaagt tacatcaaaa ccttcgagca gccaaagggc tgacagccat
      841 aaagggactt caaagagatt acaagggagt gtgccccaag tccttgattt tgaagaagag
      901 tgtgagtggc aagtcctagc gagccaatgg ggaaatgaga ctgatgagag agctgatact
      961 gtaaaaaaag taagcctatg tgagagagat aaaaaaaaga ggacgccgcc agaaaaacaa
     1021 ggccaaaagt ggaaagaact aggtgacagt ttgacttttg gttcggcgat atctgagagc
     1081 ctgattggga cagaaggaaa gaaattctat aagtgcgata tgtgctgtaa acattttaac
     1141 aaaatttccc acctaataaa ccacaggaga atccataccg gagagaagcc ccataaatgt
     1201 aaagagtgcg gaaaaggttt tatccagaga tcgagccttt taatgcacct gaggaaccac
     1261 agtggtgaga aaccatataa gtgtaatgaa tgcggtaagg ctttcagcca atctgcatac
     1321 ctcctaaacc atcaaaggat acatacagga gaaaaaccgt ataagtgtaa ggagtgcggg
     1381 aaaggtttct acagacacag tggcctaata atccacctca gaagacacag cggagagagg
     1441 ccatataagt gtaatgagtg cggtaaagta ttttcccaga atgcgtacct gatagaccac
     1501 cagagactac ataaaggaga agagccatat aagtgtaata agtgccagaa ggcttttatt
     1561 ttaaaaaaat ccctgatcct ccaccagaga attcacagcg gtgaaaagcc atataagtgt
     1621 gatgaatgcg gaaagacttt tgcccaaacc acgtacctaa ttgaccacca aagattacat
     1681 tcggctgaaa acccatataa gtgtaaagaa tgtggaaaag tttttatcag atcaaagagc
     1741 ctcctcctac accaaagagt acacactgag aaaaagactt tcgggtgtaa aaagtgcggg
     1801 aaaatttttt ccagtaagag taattttata gaccataaga ggatgcacag cagagaaaag
     1861 ccatacaagt gcactgaatg cgggaaggca tttacccaat cggcatacct ttttgatcac
     1921 caaagactcc ataacggtga aaagccgtat gagtgtaatg aatgtgggaa agtattcatc
     1981 ctaaagaaat ccctaattct ccaccagaga ttccatactg gtgaaaacct gtatgaatgt
     2041 aaagactgcg gaaaagtatt tggttcaaac agaaacctga ttgaccatga gaggctccat
     2101 aatggagaaa agccatatga gtgcagagag tgtggtaaga cgtttataat gtcgaaatct
     2161 tttatggtac accaaaagct ccacacccaa gaaaaggcat ataaatgtga agactgtggg
     2221 aaggcttttt catataacag cagtctcctt gtacacagga ggatacacac cggagaaaag
     2281 ccttttgaat gttctgaatg tggcagagct ttcagcagta acagaaattt gatagagcac
     2341 aagaggatcc attctggaga gaaaccatat gaatgtgatg agtgtgggaa gtgctttatc
     2401 ctgaaaaaga gtttaattgg ccaccagaga atccacacga gagagaaatc atataagtgt
     2461 aatgactgcg ggaaagtttt ttcatacaga tcaaacctaa tagcccacca aagaatacat
     2521 accggtgaaa aaccatatgc gtgcagtgag tgtggaaagg gttttacata taacaggaat
     2581 ttaattgaac accagaggat tcacagtgga gaaaagacat atgaatgcca tgtgtgcaga
     2641 aaagtcctga catccagcag gaacctaatg gtccaccaga gaatacacac aggtgaaaag
     2701 ccatataagt gtaatgaatg tggtaaagat ttttcccaaa ataagaacct tgttgtacac
     2761 cagagaatgc acaccggtga gaagccatat gaatgtgata agtgcaggaa gagtttcact
     2821 tcaaaaagaa accttgtagg ccaccaaagg atccacactg gagaaaagcc atatggatgt
     2881 aatgactgtt caaaagtttt cagacagaga aaaaacctga ctgtccacca aaaaatacat
     2941 actgatgaaa agccctgtga gtgtgatgtt tcagagaaag aattcagcca gacatcaaac
     3001 ctccacctcc aacaaaagat ccacacaata gaagaattca gctggctcca aaacacaaac
     3061 gaatcgaaga tagagatcca aaagatatat acccagcttt cttgtacaaa gttggcatta
     3121 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     3181 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     3241 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     3301 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     3361 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
     3421 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     3481 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     3541 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     3601 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     3661 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     3721 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     3781 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     3841 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     3901 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     3961 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     4021 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     4081 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     4141 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     4201 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     4261 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4321 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4381 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4441 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4501 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4561 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4621 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4681 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4741 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4801 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4861 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4921 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4981 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5041 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5101 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5161 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     5221 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     5281 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     5341 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     5401 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     5461 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     5521 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     5581 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggctta
//
