LOCUS       sequence-19156-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf73
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             647..688
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             698..715
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(738..755)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 accgaattcc agctgaccac catgggacgc cgcgtttgtt attactccaa tgggtgtgtt
       61 gactataaca gatacattgt atgatgatct cgatatctca atcatggact ttataggacc
      121 atacattata ggtaacataa aaactgtcca aatagatgta cgggatataa aatattccga
      181 catgcaaaaa tgctacttta gctataaggg taaaatagtt cctcaggatt ctaatgattt
      241 ggctagattc aacatttata gcatttgtgc cgcatacaga tcaaaaaata ccatcatcat
      301 agcatgcgac tatgatatca tgttagatat agaagataaa catcagccat tttatctatt
      361 cccatctatt gatgttttta acgctacaat catagaagcg tataacctgt atacagctgg
      421 agattatcat ctaatcatca atccttcaga taatctgaaa atgaaattgt tgtttaattc
      481 ttcattctgc atatcagacg gcaatggatg gatcataatt gatgggaaat caatagtaat
      541 tttttatcat aagcaattcc cggggatccg aataaagggt caagacaatt ctgcagatat
      601 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
      661 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
      721 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgcc
//
