LOCUS       sequence-191577-        4559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF233 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF233
FEATURES             Location/Qualifiers
     source          1..4559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2014..2113)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2130..2149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2131..2149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2154..2170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2154..2170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2272..2291)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2283..3092
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2350..2369)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3239..3827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3728..3747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3981..3998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4157..4184)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4276..4362)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4411..4433
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4425..4442
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4426..4442
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4458..4557
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgacaaaat tccaagagat ggtgactttt aaagatgtgg ctgttgtttt cacgagggaa
       61 gaacttggcc tcctagattt ggcccaaaga aagctatacc aagatgtaat gctagaaaat
      121 ttcaggaacc tcctcagtgt cggataccag ccttttaaac ttgatgtaat cctccagctt
      181 ggaaaagaag acaaattgag gatgatggag actgaaatcc aaggagatgg gtgttctggc
      241 cataaaaacc aaaatgaaat agacaccctc caagaagtga gactgagatt cctatcatat
      301 gaggacctaa tctgctggca gatctgggaa caattcactt caaaactgac atcaaaccaa
      361 gacctgataa taaacctaca aggaaagaga tcgaaactcc tgaaacaagg agacagcccg
      421 tgccaagtct ggactggtga aagcagccaa gtttcagaag atgagaatta tgtaataaaa
      481 ctccaaggtg aatcgagtaa ttcgataaag aaccaagaac tgcccctaag gacgacctgg
      541 gatttctgga ggaaaatgta cctcagagag ccccaaaatt accaatcaag gtgccaacaa
      601 atagatgtga agaataaatt gtgtaagtgt gaccactgtg tcagacagag aattgcccac
      661 caacatgatg accatggtgt ccataagaga gaaaaggctt tcagccataa taactgcggg
      721 aaagactgtg taaaagaatc ttcccaacac agtataatcc aaagtggaga acagaccagt
      781 gatgaaaatg ggaaaggttt gagtgttggt tcaaacctag aactccacca acaattacac
      841 ctgagagata aaccccatgt gaatgttgaa tacgggaaag gaattggata ttcgagtggt
      901 ttgccgagac accagtgttt ccatattggt gaaaagtgtt acagaaatgg agattctgga
      961 gaaggatttt cacaaggatc acacctccag ccccaccaga gagtttcaac tggagaaaac
     1021 ctatacagat gccaagtata tgccagaagt tcgaaccaaa acagctgcct acccagccat
     1081 gaattaaccc acccaggtga aaaattgtgc acatgtggca gatgtggtaa aggtttccac
     1141 cattcccttg attttgatat acactgtgta gattcggccg gtgagagggc atgtaaatgt
     1201 gatgtatatg ataagggttt cagccagaca tcccaattac aggctcacca gagaggccac
     1261 agcagagata agacatataa gtgggaagtg agtgacagga tttttaacag gaacagtggt
     1321 ttacaccaga gagtacacac cggagaaaag ccatataagt gtgaagtatg tgataaaggt
     1381 ttttcgaagg catcgaacct ccaggcccat caaagaatcc atactggtga gaaaccatat
     1441 aagtgtgatg tgtgtgataa gaatttttcc agaaacagcc atttacaggc acaccagagg
     1501 gtccacacag gagaaaaacc atataagtgt gatacgtgtg ggaaagattt cagccaaatc
     1561 agccacctac aggcccacca gagagtccat aaaggtgaga agccatataa gtgtgagacg
     1621 tgcggaaaag gtttttccca atcatcccat ttacaagacc accaacaagt ccataccgga
     1681 gagaacccat ataagtgtga tgtctgtgga aaaggattca gctggagcag ccatctccaa
     1741 gcacaccaaa gggtccatac aggagagaaa ccatataaat gtgaagagtg cagaaaaggt
     1801 tttatctgga attcatacct ccatgtccac cagagaatcc acactggaga gaagccatat
     1861 aagtgcggaa tgtgtggaaa gagtttttca cagacgagcc acctccaagc ccaccaaaga
     1921 gtccacaccg gtgaaaagcc gtataagtgt tttgtatgtg ggaagggttt ttcaaaatcg
     1981 agcctaagca gtgacagttc tgaatcacca tatacccagc tttcttgtac aaagttggca
     2041 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2101 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2161 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2221 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     2281 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
     2341 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     2401 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2461 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     2521 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     2581 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     2641 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     2701 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     2761 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     2821 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     2881 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2941 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     3001 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     3061 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     3121 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg
     3181 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3901 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3961 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4021 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4081 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4141 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4201 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4261 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4321 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4381 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
     4441 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
     4501 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctta
//
