LOCUS       sequence-191581-        4484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF681 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF681
FEATURES             Location/Qualifiers
     source          1..4484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1939..2038)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2055..2074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2056..2074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2079..2095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2079..2095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2197..2216)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2208..3017
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2275..2294)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3164..3752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3653..3672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3906..3923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4082..4109)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4201..4287)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4336..4358
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4350..4367
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4351..4367
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4383..4482
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggagcccc taaaattcag agatgtggcg attgaatttt ccctagaaga gtggcagtgc
       61 cttgacacga tccaacaaaa tttatacagg aatgtaatgc tagaaaatta cagaaacctt
      121 gttttccttg gaattgtggt gagtaagcct gacctaatca cgtgcctaga acaagaaaaa
      181 gagccgtgga cgagaaagag acacagaatg gtggctgaac cccctgtaat ctgttcccat
      241 tttgcccaag atttcagccc agaacaaaat ataaaagatt ctttccagaa agtcacgccc
      301 aggagatatg ggaagtgtga acatgagaac ctccaattat caaaatctgt tgatgagtgt
      361 aaagtccaaa aaggtggata taatggccta aaccagtgcc tccccactac ccaatcaaag
      421 attttccagt gtgataaata catgaaaatc ttccataagt tttcgaacct aaacggccat
      481 aaagtcaggc acaccaggaa gaagcctttt aagtataaag aatttgggaa gagtttctgc
      541 attttttcaa acctcaccca acataagatc atctgcacca gggtgaactt ctataagtgt
      601 gaagactgtg ggaaagcatt taatggcagt tcgattttca caaaacataa gagaatccat
      661 attggtgaga aatcgtacat ctgtgaagag tgtgggaagg cgtgtaacca atttacaaac
      721 ctgacgacac ataagataat atacaccaga gataaactat ataagaggga ggagtgttca
      781 aaggctttta acctcagcag ccatattacg acccacacaa ttatacatac aggagaaaac
      841 ccatataaga gagaagaatg tgataaggct tttaaccaga gcctaaccct caccacccat
      901 aagattatcc acacgagaga aaaattgaat gaatataaag agtgtggaaa ggcttttaac
      961 cagagttcac acctgaccag acataagata atccacaccg gtgagaaacc gtataagtgt
     1021 gaagaatgtg gtaaagcttt taaccagagc agccatttga cgaggcataa aataatacac
     1081 accggagaaa aaccatacag atgtgaggaa tgcggtaagg cattcagaca atcaagccac
     1141 ctaaccacac ataaaattat ccataccggt gaaaaaccat ataagtgtga ggagtgcgga
     1201 aaagctttta ataaaagttc ccaccttacg agacataaat caatccacac aggagagaag
     1261 ccatatcagt gtgagaagtg cggtaaagca tcaaaccaaa gttcgaacct tactgaacat
     1321 aagaatatcc acacagaaga gaagccatat aaatgtgaag aatgtgggaa ggcttttaac
     1381 caattcagta acctgactac ccataaaagg atacacactg gagaaaagcc gtataaatgt
     1441 gaagagtgtg gtaaggcttt taaccaaagc agtattttga caacccataa gaggatccat
     1501 actggtgaga agagttataa gtgtgaagaa tgtggaaaag cattctacag gagttcaaag
     1561 ctcacagaac ataaaaaaat ccatacaggt gaaaagccat acacgtgtga agagtgcggg
     1621 aaggcattta accattccag ccacctggca acacataaag tgatacacac aggtgagaag
     1681 ccataccaat gtgaagaatg cgggaaagct tttaaccaat catcccacct gacaagacat
     1741 aaaagaatac atactggaga gaaaccatac cagtgtgaaa aatgcggaaa ggcttttaac
     1801 caatcttcga atttgactgg ccataagaag atccacactg gtgaaaaact gtataagcca
     1861 aagaggtgta acagtgattt tgagaacact tcgaaatttt cgaaacataa gaggaactat
     1921 gccggagaga aatcatatac ccagctttct tgtacaaagt tggcattata agaaagcatt
     1981 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2041 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2101 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2161 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     2221 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     2281 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     2341 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     2401 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     2461 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     2521 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     2581 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     2641 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     2701 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     2761 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2821 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2881 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2941 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     3001 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     3061 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     3121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3301 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3781 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3841 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3901 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3961 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4021 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     4081 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     4141 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     4201 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     4261 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     4321 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
     4381 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
     4441 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctta
//
