LOCUS       sequence-191591-        5408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF507 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF507
FEATURES             Location/Qualifiers
     source          1..5408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2863..2962)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2979..2998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2980..2998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3003..3019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3003..3019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3121..3140)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3132..3941
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3199..3218)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4088..4676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4577..4596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4830..4847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5006..5033)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5125..5211)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5260..5282
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5274..5291
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5275..5291
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5307..5406
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggaagaat cttcttctgt ggcgatgctt gtcccagata ttggtgaaca agaggcaatt
       61 ttgacggctg aatcaataat atcgccttcc cttgaaatag atgaacagag gaagacgaag
      121 cctgaccccc tgatccatgt aatccagaaa ctttcgaaaa ttgtagagaa tgaaaaaagc
      181 cagaaatgcc ttttaattgg gaaaaagagg ccgagatcga gtgcggctac ccacagccta
      241 gaaacccaag aattgtgtga aataccagca aaagtaatcc aatcgccggc ggcagatacg
      301 aggagggcag aaatgagcca gacaaatttt acgcctgata ccctggccca aaatgaagga
      361 aaggcaatga gttaccagtg ttccctctgt aaattcctga gttcaagttt ttctgtccta
      421 aaggaccata taaaacagca tggccaacaa aatgaggtga tcctaatgtg cagtgagtgc
      481 catatcacga gcagaagcca agaagaatta gaggcccatg ttgtaaatga ccatgataat
      541 gatgcaaata tccataccca atcgaaagcc caacaatgtg tatcgccgag cagcagccta
      601 tgcaggaaaa ccactgaaag aaatgaaaca atccctgata tacctgtaag tgtagataac
      661 ctccagaccc atactgtcca aacggccagt gttgctgaaa tggggaggag aaaatggtat
      721 gcatatgaac aatatggaat gtacaggtgc cttttctgca gttacacatg tggacaacag
      781 agaatgctaa aaacccatgc ctggaaacat gctggagaag tagactgttc atacccgatt
      841 tttgaaaatg agaatgagcc tttaggcctt ttagacagca gtgctgcagc ggcccctggt
      901 ggtgttgatg ctgttgtaat agcgattggt gagagtgaat tatcgatcca taacggacca
      961 tctgtccaag tccaaatatg ttcttcagaa caactatcat caagttcgcc ccttgaacaa
     1021 tctgctgaga gaggtgtcca cctttcccaa agtgtgaccc tagacccaaa tgaagaagaa
     1081 atgctagaag taataagtga tgctgaagag aacctaatcc cagacagcct cctgacatct
     1141 gcccaaaaaa taatttcttc gagcccgaat aaaaaaggcc atgtaaatgt aattgttgag
     1201 aggctccctt cggctgaaga aaccctgagc caaaagagat tcctaatgaa tacagaaatg
     1261 gaagaaggaa aagacctcag ccttacagaa gcccaaattg gaagagaagg aatggatgat
     1321 gtatacaggg ctgataagtg cactgtggat attggaggtt tgataattgg gtggagttcg
     1381 agtgaaaaga aagatgagct gatgaataaa ggtttggcca cagatgaaaa tgcaccccct
     1441 gggagaagga gaacgaacag tgaaagtttg agattacaca gtttggcagc tgaagccctt
     1501 gtcacaatgc caatcagggc tgctgaatta acgagggcaa atttaggcca ttatggtgat
     1561 ataaacctat tagaccctga cacgagccag agacaagttg acagcacttt ggctgcatat
     1621 tcaaaaatga tgagccccct aaaaaattca agtgatggcc ttacaagcct aaaccaatca
     1681 aattcaaccc ttgtagccct ccctgaagga agacaagaat tatctgatgg ccaagtaaag
     1741 actgggattt cgatgagcct ccttactgta attgagaaat taagagagag gacagaccaa
     1801 aatgcttcag atgatgatat cctgaaagag ctccaagata atgcccagtg ccagccaaat
     1861 tctgatacat ccctgagcgg aaataatgtt gtagaatata tcccgaatgc agaaagacca
     1921 tacaggtgca gactgtgcca ttacacgagt ggaaataaag ggtacataaa acaacacctc
     1981 agagtccaca gacagagaca gccataccaa tgcccaatct gtgaacacat agctgataat
     2041 tcgaaagatt tagagagcca catgatccac cactgtaaga cccggatata ccagtgcaaa
     2101 cagtgtgaag aatcattcca ttataagagc caattaagaa accatgagag agaacaacat
     2161 tccctgccag ataccctcag tatagcgact tcaaatgaac cgagaatttc cagtgacact
     2221 gcagatggaa agtgtgtcca agaagggaat aaatccagtg tccaaaaaca atacaggtgt
     2281 gatgtctgtg attatacgag cactacatat gtcggagtga gaaaccacag gagaatccat
     2341 aattcagata agccatacag atgcagtttg tgcggatatg tatgttccca ccccccgagc
     2401 ctaaaatccc atatgtggaa acacgccagt gaccaaaact ataattatga gcaagtgaat
     2461 aaggcaataa atgatgcgat atcccagagc ggcagagttt taggaaagag ccctggaaag
     2521 acccaattga aatcaagtga agagagtgct gaccctgtta ctggaagttc agaaaatgct
     2581 gtcagttctt ctgaattgat gagccaaacc ccttctgaag tccttggcac aaatgaaaac
     2641 gaaaaattga gcccgacatc gaacacatca tattccctag aaaaaatatc ttctttagcg
     2701 ccgccgagta tggaatactg tgtccttctt ttctgctgct gtatatgtgg ttttgagagc
     2761 acgtcgaaag aaaacctcct agaccacatg aaagaacatg aaggggagat tgtgaatatc
     2821 atcctaaata aagaccacaa cacggccctg aacacaaact atacccagct ttcttgtaca
     2881 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2941 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     3001 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3061 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     3121 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     3181 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     3241 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     3301 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     3361 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     3421 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     3481 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     3541 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     3601 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     3661 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     3721 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3781 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     3841 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     3901 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3961 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     4021 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4081 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4141 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4201 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4261 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4321 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4381 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4441 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4501 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4561 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4621 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4681 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4741 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4801 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4861 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4921 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4981 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     5041 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     5101 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     5161 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     5221 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     5281 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
     5341 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
     5401 caggctta
//
