LOCUS       sequence-191595-        5771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF518B transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF518B
FEATURES             Location/Qualifiers
     source          1..5771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3226..3325)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3342..3361)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3343..3361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3366..3382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3366..3382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3484..3503)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3495..4304
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3562..3581)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4451..5039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4940..4959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5193..5210
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5369..5396)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5488..5574)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5623..5645
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5637..5654
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5638..5654
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5670..5769
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgaaagata ttggacaaca attatacacc acccacctaa atggtggcca taattctttg
       61 acaatgagcc cgaaacagcc agatgcaaat ggggcaccca ggccaaacag acaagaggcc
      121 cagaccctcc tataccaagg aagtgaagct gaggcggcaa tgatgacaat agcaacgtgt
      181 gcaaagtgta agagtgtcca taaaataagc ctccaagacc tccaaaaagg aactggaaaa
      241 gatggaatgt atgtatgttt ccaatgcagt ttaggggcag cacccccaaa tttccatttt
      301 gtcagtaata acagcagtgc cacccatgta gggaataaaa cggagaactt cagcagttct
      361 gtgaacagta aatttaaagt gaggaacttt aagcccggga aatactactg tgataagtgc
      421 aggttttcga cgaaagaccc cctccaatat aagaaacaca ccctccaaca cgaagagata
      481 aaatttatct gcagccactg ttcatatatt tcgtacacaa agggagagtt ccaaagacac
      541 cttgtaaagc acactggaat tttcccatac cagtgtgaat actgcgatta tggggcgatc
      601 agaaatgatt atattgtaaa acacacaaag agggtccatg agagggctgg agcgaaaaga
      661 cctgtaaagg ctgttgcaaa attagagcca aagaggactg gaacatcaaa acaaaaccct
      721 gaactcctaa aagcttcaaa ccccaggacg actttccaaa ataagtggag tgaccaactg
      781 agtggtttca gcctccatgc aaataaagat aagatgcata atataatgct cctcccagaa
      841 ccgaaagaat accaaaaaga cgttgtgtgc atcccgaata aaatgaccct cagtgagccg
      901 aatgaagtga acctttttga gaataagaat gttgaagtag aagtcctgag cccggcaaaa
      961 gaacctgtcc aacctggaat gcctttgact gttgtggccc cagctgagct tgttgtgcca
     1021 gcaaactgcc tggcccaatt gatagatgtg aaagttgtaa atggaaccca acagcttgtc
     1081 ctgaaattat tcccattaga agagaataac tgcctagagg ctggcagaga taatggtggg
     1141 aattcagaaa gaatggtaaa agagaaagga tcgaatgaac aagaaaaagt cctatcggca
     1201 gaaaagacaa aaagcctcac tgtagacgga aatgttggga aacttgttgg aattgacagt
     1261 ttccagccca gtgtccaaaa acaattaaag aatgtaaagt gggtcagatc gtatgacttc
     1321 ataatgccaa attcgagcgt ccacaacaat gggaagagtt tcatcaacag tgagacaatc
     1381 gaagacttcc aaaagaagaa caatttatac ccccacagga cggctttccc cagtgtggct
     1441 ttaaaaggac acagtttagc aagtgtattt aagaacagtg ttttaagaag ccttggggct
     1501 gcatcaaacc ctttcccata taaggcggct gtctgttttg ctgagagtgg aagaaacctc
     1561 cattcatcat cccaacaact tttgcctttt gcagcgagcc cagccacgtg ttctttttct
     1621 ggagaaaaag gccttctccc tgtcagcgag aatgatttag agagcacatc gaaagtaaat
     1681 atccctgtaa aagttgtgag ttcaaacaga aaacaagagg ataaccagac agaggaacac
     1741 aaggctgtca gcactgttgg acaaatttct tcacaacata agagtgaata cctccatata
     1801 aatatcactg gtgaagacag gagccaacaa cctggtgata agcccctaga attgaagaat
     1861 tctgagagga caaataacac gaatgatggg cctgtaatat caagtgtttt ttctttatcg
     1921 agtgggagtg agaatgtccc tgaagggata aagtggaatt cgagcacttc gaaaataaaa
     1981 tcgattgagc tcctcagaag aaaaattgcc cagctaattg agagctgtgg aaagccatct
     2041 tccctggcat caaattctgc ccacagaaga agtgtaggcc aggcttcaaa agggacatca
     2101 aaggccactt ctgaaggaat ccaagaaata aatgtatctt taacaggttt aggccacagc
     2161 actggcaccc tacaaaagcc accaaatgat ggtggaatca ctggaaacag acaacttacc
     2221 caccaacaaa tatatcccca ttttgcagat ggatcaaaca ggaaaacgaa atcgagagtg
     2281 gccagaaaag cccatgttgc gacccctgtt ttaataccga aaggagctgt cctcagagtt
     2341 ttgaacagtt cagaaaatgc acatataata gaagccacat gtgaagcccc tgtatcaatc
     2401 ccgtgcagtg agagacaatt aataaaacct gtacctttct gccctgtaag acaagcagat
     2461 tcagatttac agcccctgag atctgaaaga gggccaatag atatgagccc aaatattgaa
     2521 acccctttga ggccaaaatt gagaaaagaa agtgctgtat gttcgacaat ccacagaaaa
     2581 actggcctcc tatatggcca acaaggatcc agtgaattaa ataaacaagg gagactcctc
     2641 agcaggagtt tatcaatcag cagaaataag acgaaacagg tccacctttc gagaaaaaag
     2701 aataagatcc aggcagaacc ttcaaggtgc ctaaaagacc cttcaatttt ccaagtagca
     2761 agacaattga gattaattgc ggcaaagcca gaccaactaa taaagtgccc gaggagaaac
     2821 cagcctgtga ttgtcctaaa ccaccctgat gttgattcgc cagaagtgac gaatgtgatg
     2881 aaagtaataa ataaatataa agggaatgtc ctgaaggttg tcctgagtga aagaacgagg
     2941 tgccaattag gaatcaggag gcaccatgtc agattgacat accagaatgc agaagaggcc
     3001 agccaaataa aaagacagat gatgctgaag atgaaattga aaaaggtcca caagaacaac
     3061 taccaagttg tagattccct ccctgatgat tccagccaat gtgtttttaa gtgctggttc
     3121 tgtggcagat tatatgaaga ccaagaagag tggatgagcc atggccagag acacctaata
     3181 gaggctacca gagactggga tgtcctatca tcaaaaggaa aatataccca gctttcttgt
     3241 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     3301 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     3361 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     3421 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     3481 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     3541 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     3601 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     3661 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     3721 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     3781 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     3841 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     3901 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     3961 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     4021 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     4081 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     4141 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     4201 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     4261 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     4321 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     4381 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     4441 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4501 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4561 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4621 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4681 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4741 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4801 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4861 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4921 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4981 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5041 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5101 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5161 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5221 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5281 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     5341 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     5401 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     5461 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     5521 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     5581 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     5641 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
     5701 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
     5761 aagcaggctt a
//
