LOCUS       sequence-191599-        4235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF579 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF579_isoform2
FEATURES             Location/Qualifiers
     source          1..4235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1690..1789)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1806..1825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1807..1825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1830..1846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1830..1846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1948..1967)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1959..2768
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2026..2045)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2915..3503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3404..3423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3657..3674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3833..3860)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3952..4038)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4087..4109
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4101..4118
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4102..4118
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4134..4233
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggacccac aaccaccgcc accagcccaa ggatcacccc cacacagagg aagaggaaga
       61 ggaagaggca gagggagagg aagaggaaga gggaggggaa gaggtggtgc tggtgcgccc
      121 agagcacctt tgccatgccc tacgtgtgga agattattca gattcccata ttacctcagc
      181 agacacagat taagccattc tggcctaagg ccccatgcat gccctttgtg cccgaaggct
      241 ttcaggaggc ctgcccattt atccagacac ctaagaggcc atgggcccca accaccttta
      301 agatgtgctg cctgccccag gactttccct gagccggccc aattgagaag acacctggcc
      361 caagaacatg ctggaggtga agtagaattg gcaatagaaa gagtagcaaa agagactgca
      421 gagccaagct ggggacccca agatgaaggc agtgaacccc ctacaacggc tgctgctggg
      481 gcgactgaag aagaggctgt tgcagcatgg ccagagacat ggcctgctgg agagccttcg
      541 actttagcgg cacccacttc agctgctgag ccgagagaat cagagagtga agaagcagaa
      601 gctggtgctg cagaattaag ggctgaattg gctttagcag ctggaagaca agaagaaaaa
      661 caagtcctcc tccaggcaga ctggaccctt ttgtgtttga ggtgcagaga agcttttgca
      721 acaaaaggag aattaaaagc ccacccctgc ctgaggcctg aaggtgaaca agaaggagaa
      781 ggaggccccc cgcccaggcc aaagagacac cagtgttcaa tatgtttaaa ggcttttgcg
      841 aggccgtgga gcctatcaag acacaggctt gtccattcga cagacagacc ttttgtctgc
      901 ccagactgtg gccttgcttt cagattggct tcatacctca gacaacacag aagagtccat
      961 ggccccctat cccttttggc gcctttgcct gcggctggaa aaaaagatga taaggccagt
     1021 ggagcgagaa attcagcaaa agggccagaa ggtggagaag gtgctgagtg tggtggtgcg
     1081 agtgaaggtg gtgaaggcca aaatggtgga gatgcagcgc cagcaagacc cccggctgga
     1141 gaaccgagat tctggtgccc tgaatgtgga aaaggtttca gaagaagggc ccatttaaga
     1201 caacatggtg taacccacag tggtgcaaga cctttccagt gtgtgagatg ccagagagaa
     1261 tttaagagat tagctgacct ggccagacat gcccaagtcc atgcaggagg acctgcaccc
     1321 cacccgtgcc ccagatgccc gaggagattt tccagagcat acagcctcct aagacaccaa
     1381 agatgccaca gagctgaatt agaaagggca gctgctttac aagccctcca agcccaagca
     1441 cccacatcac cgccgccgcc cccaccccct ttaaaagcag aacaagagga agaaggcctc
     1501 cctttacccc tagcaaatat aaaagaagaa ccccccagcc ctggcacacc cccccagagc
     1561 ccgccagccc cacctgtttt tttaagtgca tcgtgttttg attcccaaga ccattcggct
     1621 tttgaaatgg aagaagaaga agtagacagt aaagcccatt tgagaggcct tggtggttta
     1681 gcatcatata cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     1741 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     1801 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     1861 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     1921 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     1981 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     2041 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     2101 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     2161 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     2221 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     2281 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     2341 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     2401 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     2461 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     2521 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     2581 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     2641 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     2701 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     2761 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     2821 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2881 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2941 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3001 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3061 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3121 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3181 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3241 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3301 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3361 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3421 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3481 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3541 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3601 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3661 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3721 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3781 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3841 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3901 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     3961 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4021 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     4081 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     4141 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     4201 ttataatgcc aactttgtac aaaaaagcag gctta
//
