LOCUS       sequence-19160-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf77
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             635..676
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             686..703
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(727..744)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ccgaattcca gctgaccacc atgaacaaac ataagacaga ttatgctggt tatgcttgct
       61 gcgtaatatg cggtctaatt gttggaatta tttttacagc gacactatta aaagttgtag
      121 aacgtaaatt agttcataca ccatcaatag ataaaacgat aaaagatgca tatattagag
      181 aagattgtcc tactgactgg ataagctata ataataaatg tatccattta tctactgatc
      241 gaaaaacctg ggaggaagga cgtaatgcat gcaaagctct aaatccaaat tcggatctaa
      301 ttaagataga gactccaaac gagttaagtt ttttaagaag cattagacgc ggatattggg
      361 taggagaatc cgaaatatta aaccagacaa cctcatataa ttttatagct aagaatgcca
      421 cgaagaatgg aactaaaaaa cggaaatata tttgtagcac aacgaatact cccaaactgc
      481 attcgtgtta cactatataa caattacact acatttttat cataccacta cttcggttag
      541 gcaattcccg gggatccgaa taaagggtca agacaattct gcagatatcc agcacagtgg
      601 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
      661 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt taacccgctg
      721 attcagcctc gactgtgcct tctagtgc
//
