LOCUS       sequence-19161-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf78
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             540..581
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             591..608
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(631..648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    637..748
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccagtaccct tcaccgaatt ccagctgacc accatggctg aatggcataa
       61 aattatcgag gatatctcaa aaaataataa gttcgaggat gccgccatcg ttgattacaa
      121 gactacaaag aatgttctag ctgctattcc taacagaaca tttgccaaga ttaatccggg
      181 tgaaattatt cctctcatca ctaatcgtaa tattctaaaa cctcttattg gtcagaaata
      241 ttgtattgta tatactaact ctctaatgga tgagaacacg tatgctatgg agttgcttac
      301 tgggtacgcc cctgtatctc cgatcgttat agcgagaact cataccgcac ttatattttt
      361 gatgggtaag ccaacaacat ccagacgtga cgtgtataga acgtgtagag atcacgctac
      421 ccgtgtacgc gcaactggta attaagcaat tcccggggat ccgaataaag ggtcaagaca
      481 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag
      541 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
      601 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
      661 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      721 tttcctaata aaatgaggaa attgcatcgc atgtctg
//
