LOCUS       sequence-19163-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf80
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             383..424
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             434..451
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(474..491)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    480..591
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttggtaccga gctcggatcc agtacccttc accgaattcc agctgaccac catgctatta
       61 gaaatggata aaatcaaaat tacggttgat tcaaaaattg gtaatgttgt taccatatcg
      121 tataacttgg aaaagataac tattgatgtc acacctaaaa agaaaaaaga aaaggatgta
      181 ttattagcgc aatcagttgc tgtcgaagag gcaaaagatg tcaaggtaga agaaaaaaat
      241 atgatcgata ttgaagatga cgatgatatg gatgtagaaa gcgcataagc aattcccggg
      301 gatccgaata aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag
      361 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
      421 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
      481 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      541 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
      601 gagtaggtgt catt
//
