LOCUS       sequence-191639-        4418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF805 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF805
FEATURES             Location/Qualifiers
     source          1..4418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1878..1977)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1994..2013)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1995..2013)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2018..2034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2018..2034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2136..2155)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2147..2956
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2214..2233)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3103..3691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3592..3611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3845..3862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4021..4048)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4140..4226)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4275..4297
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4289..4306
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4290..4306
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4322..4418,1..3)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggcaatgg ccctaactga cccggcccaa gtttctgtaa cttttgatga tgttgctgtt
       61 acttttacac aagaagagtg gggccagctt gacctggccc agaggaccct ataccaagaa
      121 gtaatgcttg aaaactgtgg cctccttgtc agtttaggat gccctgtccc aaggccagaa
      181 ttaatatacc atttagaaca tggccaagaa ccctggacga gaaaagaaga cctatcccaa
      241 ggtacctgcc ctggagataa aggaaaacca aaaagtacag aacctacgac gtgtgaatta
      301 gccctcagtg aaggaatttc tttctggggc caattaaccc aaggagcatc tggagacagc
      361 caattaggcc aaccaaaaga ccaagacggt ttttctgaaa tgcaaggaga gagattgagg
      421 cctggcctag attcccaaaa agaaaaattg cctggaaaaa tgagcccaaa acatgatggt
      481 ttaggaacgg cagattctgt gtgttccaga ataatccaag acagagtcag ccttggagat
      541 gatgtccatg actgtgattc ccatggatct gggaaaaacc ctgtgatcca agaggaagag
      601 aatattttta agtgcaatga gtgcgagaag gtttttaata agaagaggct cctggccaga
      661 catgagagaa tacattccgg tgtaaagcca tatgagtgca ctgaatgcgg taaaactttc
      721 agtaagagta catacctttt acaacaccat atggtccaca caggtgagaa gccatataag
      781 tgtatggagt gcggtaaggc atttaacagg aagagccacc ttacacaaca ccagaggatc
      841 cattccggag agaaaccata taaatgttca gagtgtggaa aagcattcac acacaggagc
      901 acttttgttc tccataacag atcccacacc ggtgagaaac cttttgtctg taaagaatgt
      961 ggaaaggcat tcagagacag acctggtttt atcagacact acataatcca cagtggagaa
     1021 aacccatatg agtgttttga gtgcggaaaa gtttttaaac acagatcgta cctgatgtgg
     1081 caccaacaga cccatactgg tgagaagcca tatgagtgtt cagaatgcgg aaaggctttc
     1141 tgtgagagtg ctgctttaat ccaccattat gtgatacaca ccggagaaaa gccttttgaa
     1201 tgcctagagt gtggtaaggc ttttaaccac aggagttatt taaagagaca ccaaaggata
     1261 cacactggtg aaaaaccata tgtgtgcagt gaatgtggta aagctttcac ccactgttcg
     1321 acttttatcc tccataaaag ggcccataca ggagaaaaac catttgaatg taaagagtgc
     1381 gggaaagctt tttcaaacag ggcagacctg ataagacatt tttcaatcca cactggagag
     1441 aagccgtatg aatgtatgga atgcgggaag gcttttaaca gaaggagtgg tttaaccaga
     1501 caccagagaa tccattcagg agagaagcca tatgagtgta ttgaatgtgg gaagactttc
     1561 tgctggagta caaacctaat cagacattcg ataatccata ccggtgaaaa gccatatgaa
     1621 tgttctgagt gtgggaaagc attttccaga agttcttccc tcacccaaca ccaaagaatg
     1681 catactggca gaaacccaat aagtgtcact gatgttggaa ggcctttcac atctggccaa
     1741 acgagtgtaa atatccaaga actcctcctt ggaaaaaatt tcctaaatgt gacgacagaa
     1801 gaaaacctcc tccaagaaga agcatcatat atggcgagtg acaggacata ccaaagagag
     1861 acgccccaag taagttccct caactttctt gtacaaagtt ggcattataa gaaagcattg
     1921 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     1981 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2041 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2101 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2161 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2221 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2281 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2341 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2401 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2461 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2521 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2581 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     2641 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     2701 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2761 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     2821 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     2881 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     2941 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3001 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3241 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3421 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3481 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3541 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3601 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3661 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3721 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3781 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3841 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3901 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     3961 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca
     4021 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
     4081 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
     4141 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     4201 tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct
     4261 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc
     4321 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
     4381 atgctttttt ataatgccaa ctttgtacaa aaaagttg
//
