LOCUS       sequence-19164-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf81
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             524..565
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             575..592
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(615..632)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    621..732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttggtaccga gctcggatcc agtacccttc accgaattcc agctgaccac catgggctgt
       61 ttgtataata gaccacgata atatcagagg agttatttac tttgaaccag tccatggaaa
      121 agataaagtt ttaggatcag ttattggatt aaaatccgga acgtatagtt tgataattca
      181 tcgttacgga gatattagtc aaggatgtga ttccataggc agtccagaaa tatttatcgg
      241 taacatcttt gtaaacagat atggtgtagc atatgtttat ttagatacag atgtaaatat
      301 atctacaatt attggaaagg cgttatctat ttcaaaaaat gatcagagat tagcgtgtgg
      361 agttattggt atttcttaca taaatgaaaa gataatacat tttcttacaa ttaacgagaa
      421 tgcgtttgag caattcccgg ggatccgaat aaagggtcaa gacaattctg cagatatcca
      481 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
      541 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
      601 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
      661 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
      721 ggaaattgca tcgcattgtc tgagtag
//
