LOCUS       sequence-191651-        3773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF735 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF735
FEATURES             Location/Qualifiers
     source          1..3773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1233..1332)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1349..1368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1350..1368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1373..1389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1373..1389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1491..1510)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1502..2311
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1569..1588)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2458..3046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2947..2966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3200..3217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3376..3403)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3495..3581)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3630..3652
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3644..3661
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3645..3661
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(3677..3773,1..3)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggcaaaaa ggcctggccc gcctgggagc agagagatgg gccttttgac tttcagagat
       61 atagcaattg aattcagttt ggcagagtgg cagtgccttg accatgccca acaaaaccta
      121 tacagagatg taatgctaga gaattacagg aaccttttca gccttgggat gactgtatca
      181 aagccagatt taatagcgtg cctggaacaa aataaagagc cccaaaatat aaagagaaat
      241 gaaatggcag caaaacaccc tgtcacgtgc agccatttta accaagacct ccagcctgaa
      301 caatcaataa aagacagcct ccaaaaagta atacccagaa catatggaaa gtgtggccat
      361 gaaaacctcc aattgaagaa gtgctgtaag agagtagatg aatgtgaagt ccataaaggt
      421 ggatataatg acctgaacca gtgcctatcg aacacccaga ataagatttt ccagacccat
      481 aagtgtgtga aggttttttc gaagttttca aacagtaaca ggcataatgc gagatacact
      541 ggaaagaagc acctgaagtg taaaaaatac gggaagagtt tctgcatgtt cagccaccta
      601 aaccaacacc agatcatcca cacgaaagaa aaatcatata agtgtgaaga gtgtggaaaa
      661 agttttaacc acagttcttc tggcacgaca cataagagga tcctaactgg agagaagcca
      721 tacaggtgtg aagaatgtgg aaaggctttc agatggcctt caaacctaac aagacataaa
      781 aggatacata caggagaaaa gccatatgca tgtgaagaat gtggccaagc tttcaggaga
      841 tcttccaccc tgacaaacca taagagaata catactggtg agaggccata taaatgtgag
      901 gagtgtggga aagctttttc tgtatcttcg gccctaatat accataaaag aatccatacc
      961 ggagaaaaac catacacctg tgaagaatgc gggaaggcat ttaactgttc cagcacccta
     1021 aaaacccata aaataatcca cacaggtgaa aagccatata cgtgtgaaga atgtgggagg
     1081 acttttaact gcagttcgac tgtaaaggca cataaaagaa tacacactgg tgaaaaaccg
     1141 tataagtgtg aggaatgtga taaggctttt aagtggcaca gcagcctggc aaaacataaa
     1201 attatccaca ccggagagaa accatataag tgtaacaact ttcttgtaca aagttggcat
     1261 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1321 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     1381 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1441 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1501 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1561 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1621 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1681 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1741 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1801 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1861 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1921 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1981 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2041 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2101 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2161 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2221 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2281 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2341 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     2401 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     3301 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
     3361 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
     3421 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
     3481 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     3541 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
     3601 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
     3661 tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
     3721 acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag ttg
//
