LOCUS       sequence-191683-        4637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF234 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF234
FEATURES             Location/Qualifiers
     source          1..4637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2097..2196)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2213..2232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2214..2232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2237..2253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2237..2253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2355..2374)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2366..3175
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2433..2452)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3322..3910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3811..3830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4064..4081
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4240..4267)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4359..4445)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4494..4516
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4508..4525
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4509..4525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4541..4637,1..3)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgaccactt ttaaagaggg cctgacattt aaagatgtgg ctgttgtttt cactgaagaa
       61 gaactcggcc ttttagaccc tgtccaaagg aacctatacc aagatgtaat gctagagaat
      121 ttcagaaacc tcctgagtgt tggccaccac ccttttaaac atgatgtttt cctcctagag
      181 aaagagaaga agctggatat aatgaagacg gcgacccaga gaaaaggaaa atcggcagat
      241 aagatccaga gtgaagttga aactgtccct gaggctggca gacatgaaga actatactgg
      301 ggccaaatct ggaaacaaat agcgagtgac ctgataaaat atgaagattc aatgatcagc
      361 atatcgagat tccccagaca aggtgaccta tcgtgccaag tgagagctgg cctatacacc
      421 acccacactg gccagaaatt ttaccaatgt gatgagtata aaaagagttt cacggacgtt
      481 tttaattttg acctccacca acaactccat tccggtgaaa agagccacac ctgtgatgaa
      541 tgtgggaaga gtttctgtta tatttcggcc ctccatatcc accagagagt tcatatggga
      601 gaaaagtgtt ataaatgtga tgtatgcggg aaagaatttt cccagagcag ccacctccag
      661 acccaccaaa gagtacatac tgtagaaaaa ccttttaaat gtgttgagtg tggtaaaggt
      721 ttcagcagga gaagcacttt aactgtccac tgtaaactcc acagcggaga gaaaccatat
      781 aactgtgagg aatgtggcag ggcttttata catgcttccc atttacaaga acaccagagg
      841 atccataccg gtgagaaacc atttaagtgt gacacgtgcg gaaaaaattt caggaggaga
      901 tctgctttaa ataaccactg tatggtccac actggtgaaa agccatataa gtgtgaagac
      961 tgcgggaagt gtttcacgtg ttcttcaaac ctcaggatac accagagagt ccatactggt
     1021 gagaagccgt ataagtgtga agaatgcggt aagtgtttta tccagccttc ccaattccaa
     1081 gcccacagga gaatacacac tggagagaaa ccatatgtgt gtaaagtctg tgggaaaggt
     1141 tttatatatt cgagcagttt ccaagcacac caaggtgtcc atacaggaga gaaaccatat
     1201 aagtgtaatg agtgcggaaa atctttcagg atgaagatcc attaccaagt ccaccttgtt
     1261 gtacacaccg gagaaaagcc atataagtgt gaagtctgcg gaaaggcttt cagacaatca
     1321 tcatacctaa aaatacacct aaaggcccac agtgtccaaa agccatttaa atgtgaagag
     1381 tgtggccaag gttttaacca aagcagcaga ttacaaatcc accaattaat ccacacaggg
     1441 gagaagccat ataaatgtga ggagtgtgga aaaggatttt caagaagggc tgacctaaag
     1501 atccactgca gaatacatac tggagaaaaa ccgtataact gtgaagagtg cggtaaagtt
     1561 ttttcccaag catcccacct tttgacacac cagagagtac acagtggaga gaagcctttt
     1621 aagtgtgaag agtgtgggaa atctttttcg aggagtgccc acctacaggc ccaccagaaa
     1681 gtacacacag gtgaaaaacc atataagtgc ggagaatgtg gaaagggttt taagtggagc
     1741 ctaaacctag atatgcacca aagggtccat acgggtgaaa agccatacac atgtggtgaa
     1801 tgcggaaaac atttcagcca agccagttcc ctacaattac accaatctgt ccacacggga
     1861 gagaaaccat ataagtgtga tgtgtgcgga aaagttttca gcagatcaag ccaattacaa
     1921 taccacagaa gagtccacac cggtgaaaag ccatataagt gtgagatctg tggaaagaga
     1981 ttcagctgga gatcgaacct tgtgagccac cataaaatcc acgcggctgg gactttttat
     2041 gaaaatgatg aaaattcaaa aaatatcagg gaattgagtg aaggtggttc gagcacgagc
     2101 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2161 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2221 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2281 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     2341 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     2401 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     2461 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     2521 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     2581 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     2641 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     2701 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2761 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     2821 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2881 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     2941 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     3001 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     3061 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     3121 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     3181 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     3241 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3301 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3361 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3421 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3481 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3541 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3601 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3661 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3721 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3781 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3841 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3901 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3961 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4021 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4081 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4141 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4201 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     4261 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     4321 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     4381 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     4441 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     4501 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
     4561 actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
     4621 tttgtacaaa aaagttg
//
