LOCUS       sequence-191703-        3743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF587B transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF587B
FEATURES             Location/Qualifiers
     source          1..3743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1203..1302)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1319..1338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1320..1338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1343..1359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1343..1359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1461..1480)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1472..2281
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1539..1558)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2428..3016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2917..2936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3170..3187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3346..3373)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3465..3551)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3600..3622
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3614..3631
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3615..3631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(3647..3743,1..3)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggctgttg tggcgacttt gagattatcg gcccaaggga ctgtaacttt tgaagatgtt
       61 gctgtaaaat ttacccaaga agagtggaac ctcctatcag aggcccaaag gtgtttatac
      121 agagatgtca cccttgaaaa ccttgcccta atgagcagcc ttgggtgctg gtgtggtgta
      181 gaagatgagg cagcgccttc aaaacaatca atatatatcc agagagaaac ccaagtaagg
      241 acgcctgtta ctggtgtatc cccaaaaaag gcccacccct gtgaaatgtg tggaccaatt
      301 ttaggtgata tcctccatgt tgcagaccac caaggtaccc accataaaca aaaattacac
      361 aggtgtgagg cgtggggaaa taagctatat gattccggga atttccacca acaccagaat
      421 gaacatattg gtgagaagcc atacagaggc agtgttgaag aggccctttt tgtaaaaagg
      481 tgtaaactcc atgtaagtgg tgagagcagt gttttttctg aaagtggaaa agatttcctg
      541 ccaagaagtg gcctcctcca acaagaggct tcccatactg gagaaaaatc aaattcaaaa
      601 actgagtgtg tttccccttt ccaatgtggt ggggcccatt acagccacgg tgacagtatg
      661 aaacatttca gcacaaaaca tatcctgagc caacaccaaa ggctcctgcc gagagaagaa
      721 tgttatgtct gctgtgaatg cggaaaaagt ttttcgaaat atgtgagttt cagtaaccac
      781 cagagagtcc attctggaaa gaggccatat gagtgcggag agtgtgaaaa atctttcagc
      841 caaaagagca gtttaatcca acaccaacaa ttccacaccg gtggaaagcc atatgggtgt
      901 gaagagtgtg gaaaatattt cagcctagaa ggatacctca gaagacacca aaaagtccat
      961 gctggaaaag gaccatatga atgtggagag tgcgggaaga gtttttcttc aaatgtaaac
     1021 ctaaagagcc accagaggat ccacacagga gaaagaccat ataagtgtgg tgagtgtgag
     1081 aaaagtttca gcagaaagcc gagcctatca taccaccaaa gatttggcag gccgaggtgg
     1141 gtagaccata aagacagaaa agaatttaag acgagccttg gaaatattgt aaagagctgc
     1201 cttttcaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1261 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1321 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1381 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     1441 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     1501 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     1561 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     1621 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     1681 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     1741 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     1801 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     1861 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     1921 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     1981 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2041 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2101 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2161 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2221 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2281 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     2341 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3121 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3181 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3241 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3301 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
     3361 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     3421 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     3481 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
     3541 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
     3601 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt
     3661 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
     3721 gccaactttg tacaaaaaag ttg
//
