LOCUS       sequence-191705-        3890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF222 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF222
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1350..1449)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1466..1485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1467..1485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1490..1506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1490..1506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1608..1627)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1619..2428
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1686..1705)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2575..3163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3064..3083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3317..3334
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3493..3520)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3612..3698)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3747..3769
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3761..3778
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3762..3778
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(3794..3890,1..3)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggcgaaat tatatgaggc tgtcactttt aaagatgtgg ctgtaatttt cactgaagaa
       61 gaacttggcc tcctagaccc ggcccaaaga aaactataca gagatgtaat gctagaaaat
      121 ttcagaaacc tcctcagtgt tggaggaaaa atccaaactg aaatggagac tgtgccagaa
      181 gctggaaccc atgaagaatt ttcgtgtaaa cagatctggg aacaaatagc gagtgacctc
      241 accagatccc aagacaccac aatttcaaat tcccaactat ttgaacaaga tgataacccg
      301 agccaaataa aggccagatt gagtactgtc catacgagag agaagccttt ccaaggagag
      361 aactgtaaac aatttttcag tgatgtcagt ttttttgacc tcccccaaca actatattct
      421 ggagagaaat cccacacctg tgatgagtgc ggaaaaagtt tctgttatat cagtgccctc
      481 catatccacc agagagtcca tatgggtgta aagtgttata aatgtgatgt atgtgggaaa
      541 gaattcagcc aatcatcaag attacagacc caccaaagag tccacacagg agaaaagcct
      601 tttaagtgtg aacaatgtgg aaaaggtttc aggtgcagaa gtgccctaaa agtccactgt
      661 aagctccata tgagggaaaa accatataac tgtgagaagt gtgggaaggc tttcatgcat
      721 aatttccaat tacaaaaaca ccacaggatc cacactggtg agaagccatt taagtgtgaa
      781 atatgcggga aatctttctg cctcagatct tccctaaaca gacactgtat ggtccacacg
      841 gctgaaaagc tatataaatc agaaaaatat ggcagaggtt ttattgacag gctagacctc
      901 cataaacacc agatgatcca catgggccaa aagccgtata actgtaaaga gtgtggaaaa
      961 tcttttaaat ggagttcata cctccttgta caccagaggg tacacaccgg tgaaaagcca
     1021 tataagtgtg aagaatgcgg aaaaggatat atttcaaaga gtggcctaga tttccaccac
     1081 agaacccata caggagagag atcatataac tgtgataact gcgggaagag tttcagacat
     1141 gcatcttcaa tcctaaacca taaaaaactc cactgccaga gaaaaccttt aaagtgtgag
     1201 gactgtggaa aaagacttgt ctgcagatca tactgtaaag accaacaaag agatcacagt
     1261 ggtgaaaacc cttcaaaatg tgaagactgc ggaaagagat ataagaggag gctgaacctg
     1321 gatataatcc tttccctttt cctaaatgat atcaactttc ttgtacaaag ttggcattat
     1381 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1441 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     1501 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1561 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1621 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1681 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1741 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1801 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1861 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1921 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1981 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2041 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2101 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2161 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2221 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     2281 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2341 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2401 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     2461 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     2521 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2701 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     3361 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     3421 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
     3481 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
     3541 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
     3601 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     3661 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     3721 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     3781 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
     3841 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg
//
