LOCUS       sequence-191707-        4226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF879 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF879
FEATURES             Location/Qualifiers
     source          1..4226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1686..1785)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1802..1821)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1803..1821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1826..1842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1826..1842)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1944..1963)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1955..2764
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2022..2041)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2911..3499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3400..3419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3653..3670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3829..3856)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3948..4034)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4083..4105
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4097..4114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4098..4114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4130..4226,1..3)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggcaagaa gacttttgcc ggcccatgtc caagaatctg tcactttcag agatgtagct
       61 gtattttttt cccaagatga atggctccac cttgattctg cccagagggc cctatacaga
      121 gaagtaatgc tagaaaatta cagcatcctt gtgagccttg gaatcctttt ttcgaagcca
      181 aaagtaatca gccaactaga acaaggtgaa gacccctgga tggtagaatc tggtgtcccc
      241 caaggggccc accttggctg ggaatccctt tttggcacga ttgtatcaaa agaggaaaac
      301 caagaggtga tgaagaagct gataattgac ggcactttcg attttaagct ggagaagaca
      361 tacatcaatg aggataagct ggaaaaacaa caggggaaga agaacaggct tttcagtaaa
      421 gtcctggtca cgataaagaa ggtatatatg aaagagagga gtttcaaagg tgtagaattc
      481 ggaaaaaacc taggcctgaa atcgagccta atcagaaagc cgagaattgt gagcagaggg
      541 aggaggccga ggagccaaca atattctgtc ctttttaaac agctcggtgt aaatactgtc
      601 aggaagtgtt ataagtgtaa tatctgcgga aagattttcc tccacagcag ttccctttca
      661 aaacaccaga ggatccatac gggagaaaaa ctatataagt gtaaagagtg caggaaagca
      721 ttttcccaat cttcatccct cacccaacac ctgagagtac atactggtga aaaaccatat
      781 atctgttctg agtgcggtaa agctttcagt ttcacgacga gtttaattgg ccaccagaga
      841 atgcatactg gagaaagacc atataagtgt aaagagtgcg ggaaaacttt taaaggttcc
      901 agcagcctaa ataaccacca gagaatccac actggagaga agccatataa gtgtaatgaa
      961 tgcggaaggg ctttttccca gtgttccagt ttaatacaac accacagaat acacacagga
     1021 gaaaagccat atgaatgcac ccaatgtgga aaagcattca catcaatttc cagattgagc
     1081 agacaccaca ggatccatac cggagaaaaa cctttccact gtaatgaatg tgggaaagtt
     1141 ttttcatacc acagtgcttt gataatccac cagagaatac ataccggtga aaagccatat
     1201 gcctgtaaag agtgtgggaa agctttttca caatcgagtg ccctaatcca acaccaaagg
     1261 atccatacag gagagaaacc atataagtgt aatgagtgtg gaaaggcatt cagctggata
     1321 tcgagattaa atatccacca caggatacac actggtgaga agccgtataa ctgcaaagaa
     1381 tgtggtaagg cattcagttc ccattctggt gtaaacaccc acagaaagat acatacgggt
     1441 gaaaagccat ataaatgtaa tgactgtgaa aaggctttta accaaagcag tgccctgatc
     1501 cagcaccaaa gaatccatac tggggagaaa ccatataact gtaaagtgtg cggaaaagct
     1561 ttcagacaaa gttcttccct aatgacccat atgaggatcc acacaggtga gaaaccatat
     1621 aagtgtaaag aatgcgggaa ggctttcagc caatcaagtt ccctcacaaa ccaccaaagg
     1681 acccataaca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     1741 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     1801 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1861 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1921 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1981 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     2041 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2101 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2161 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2221 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2281 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2341 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2401 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2461 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2521 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2581 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2641 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2701 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2761 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2821 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3541 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3601 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3661 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3721 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3781 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
     3841 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     3901 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     3961 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     4021 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     4081 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat
     4141 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
     4201 aatgccaact ttgtacaaaa aagttg
//
