LOCUS       sequence-191711-        8024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF646 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF646
FEATURES             Location/Qualifiers
     source          1..8024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(5484..5583)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5600..5619)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5601..5619)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5624..5640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5624..5640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5753..6562
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5820..5839)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6709..7297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7198..7217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7451..7468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7627..7654)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(7746..7832)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     7881..7903
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7895..7912
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7896..7912
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(7928..8024,1..3)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggaagata caccgccatc cctgagctgt tctgactgcc aaagacattt cccaagcctg
       61 ccagaattat ccagacacag agagctcctc caccccagtc caaaccaaga cagtgaagag
      121 gctgattcaa tccccagacc atacagatgc caacaatgcg gaagaggata cagacacccc
      181 ggttccctag taaatcacag gaggacacat gaaaccggcc ttttcccgtg cactacatgc
      241 gggaaagatt tttcaaaccc aatggcattg aaatcccata tgaggaccca tgcaccagaa
      301 ggcaggagga gacacaggcc ccccaggcca aaagaagcga cgccacacct ccaaggtgag
      361 actgtatcta cagacagctg gggccaaaga ttaggatcga gtgaagggtg ggaaaaccaa
      421 acaaaacaca cagaagagac tccagactgt gaatctgtgc ctgacccgag agcggccagt
      481 gggacctggg aagacctacc gaccagacaa agagaaggtt tagcttcaca ccctgggcct
      541 gaagatgggg cagatggatg gggaccttcg acaaacagtg cgagggcgcc acccctgccg
      601 ataccggcat caagcctcct atcaaaccta gaacaatacc tggctgagag tgttgtgaat
      661 tttactggtg gccaagaacc gacccaatcg ccccctgctg aagaagagag acggtataaa
      721 tgcagccagt gtggaaagac atataaacat gcaggcagcc taacaaacca cagacagagc
      781 cacaccctcg ggatataccc atgtgcaata tgttttaaag aattttcaaa tttaatggct
      841 ctgaaaaacc acagcagatt acatgcccaa tacagaccgt accactgtcc acactgcccc
      901 agagttttca gattaccaag agaactcctt gaacaccaac aatcacatga aggagaaaga
      961 caagaaccca ggtgggaaga aaaaggaatg ccgaccacaa acggccacac tgatgaaagc
     1021 agccaagacc aattaccatc agcacaaatg ctaaacggca gtgctgaatt aagtacatct
     1081 ggtgaactag aagacagcgg tttagaagaa tacaggcctt tcaggtgtgg tgactgtggc
     1141 agaacgtaca ggcatgctgg gagtttaata aaccacagga agagccacca gaccggtgta
     1201 tacccctgca gtttgtgcag taaacaatta tttaatgcgg ctgccctaaa gaaccatgtc
     1261 agagcccacc acagaccgag acaaggtgtc ggagaaaatg gccagccttc tgtaccccca
     1321 gcgcccctcc ttttagctga aactacccat aaagaagaag aagaccctac aacgaccctt
     1381 gaccacaggc catataaatg ttctgagtgt ggcagggcat acagacacag gggaagcctt
     1441 gtaaaccaca gacacagcca caggactggt gaataccagt gcagcctatg cccgaggaaa
     1501 tacccaaacc taatggccct gagaaaccat gtaagagtcc actgtaaggc agcaaggagg
     1561 agtgctgata ttggagctga aggagcgccc agtcacctta aagtagaatt accccctgac
     1621 cctgttgagg cagaagctgc gccccatact gaccaagacc atgtatgtaa acatgaagaa
     1681 gaggcgacag atatcacgcc ggctgcagat aagactgctg cccatatatg cagcatctgt
     1741 ggccttttat ttgaagatgc agaatccctt gagagacatg gcctgaccca tggggctggt
     1801 gaaaaagaga acagcaggac agaaacaaca atgagcccgc ccagggcttt tgcgtgcaga
     1861 gactgtggga aatcatacag acacagtgga tccctgataa accacagaca aacccaccag
     1921 actggagatt tcagctgcgg agcctgtgca aaacatttcc atacaatggc tgcaatgaag
     1981 aaccatttaa ggagacacag caggagaaga tccagaagac acagaaagag ggccggtggt
     2041 gccagtggtg ggagagaagc aaaactttta gcagcagaat cgtggacaag agaattagaa
     2101 gataatgaag gattagaatc cccccaagac ccatctggag aaagccccca tggtgcagaa
     2161 gggaatttag aatctgacgg tgactgcctc caagcagaaa gtgaaggaga taagtgcggc
     2221 cttgaaagag atgaaacaca cttccaaggt gataaagaaa gtggagggac cggtgaaggt
     2281 cttgaaagaa aagatgcatc attattagat aacctagata tcccaggaga agagggaggt
     2341 gggacccatt tctgtgattc cctgacaggt gtagatgaag accagaaacc agctaccggc
     2401 cagccaaaca gcagttcaca cagcgcgaat gctgtcactg gatggcaggc tggtgcagcc
     2461 catacgtgtt cagactgcgg ccattctttc ccacatgcga ctggcctcct cagccacaga
     2521 ccatgccacc cccctggaat ataccaatgt tctttgtgcc cgaaagaatt tgattctttg
     2581 ccagctttaa gatcccattt ccaaaaccac agacccggtg aggcaacttc ggcccaacct
     2641 tttttatgct gcctatgtgg aatgattttc cctggaagag ctggatacag gctacacagg
     2701 agacaggcac attccagcag cggaatgact gaaggatctg aagaagaagg agaagaagaa
     2761 ggtgtagctg aggcggcgcc ggcgagaagc ccgcctttac aattatctga agcagaactc
     2821 ctaaaccaac tccagagaga agttgaggcc ctagattcgg ccggatacgg ccatatctgc
     2881 ggatgctgtg gccagacata tgatgacctt ggatctttag aaagacacca ccagagccaa
     2941 tccagtggaa ccacggctga taaggcaccc agccccctag gtgttgctgg tgatgcgatg
     3001 gagatggttg tagattctgt tcttgaagat attgtaaatt ctgtaagtgg tgaaggcgga
     3061 gatgcaaaga gccaagaagg tgctggaaca ccccttggag acagcctgtg tatccaagga
     3121 ggtgaaagcc tactagaagc ccaacccaga cctttccgat gtaaccagtg cggaaaaaca
     3181 tacagacatg gtggcagcct tgtgaaccac agaaaaatac accaaacagg tgatttcctg
     3241 tgccctgtgt gcagcagatg ttacccgaac ctagctgcat acaggaacca cctgaggaac
     3301 catccgagat gtaaagggag tgaaccccaa gttggaccaa taccagaggc agcaggcagt
     3361 tcagaactac aagtcggccc aatccctgaa ggtggatcaa ataagcccca acatatggca
     3421 gaagaagggc ctggacaagc tgaagtagag aaactccaag aagaattaaa agttgagccc
     3481 cttgaagaag ttgcgagagt aaaagaagaa gtatgggaag agacaactgt aaaaggtgaa
     3541 gaaatagaac cgagattaga gacagcagag aaaggatgcc agactgaagc cagcagtgaa
     3601 agaccattca gctgtgaagt atgtggacgt tcatataaac acgcaggaag tttgattaac
     3661 cacagacaat cccaccaaac tggacatttt ggctgccagg cctgcagtaa aggtttttcg
     3721 aacctaatga gcctaaaaaa ccacaggaga atccatgcag acccccgcag attcagatgc
     3781 agtgaatgtg gaaaagcttt cagattgaga aaacagcttg ccagccacca aagagtacat
     3841 atggaaagga gaggtggtgg tggcacaaga aaagcaacca gagaagacag acctttcaga
     3901 tgtggccaat gcggcaggac atacagacat gccggatccc tcctaaacca caggagaagc
     3961 catgaaacag gccaatattc atgcccgacc tgcccgaaga catacagtaa cagaatggct
     4021 ttaaaagacc accaaaggct ccacagtgaa aacaggagaa gaagagccgg cagatcaaga
     4081 agaacagctg taagatgtgc tttgtgtgga agatcttttc ctgggagagg gagcctagag
     4141 agacacctca gagaacatga agagacagag agagagccag cgaatggaca aggcggtctt
     4201 gatgggacgg cagcttcaga ggcaaacctg actggaagcc aaggcctaga aacacaatta
     4261 ggtggtgctg aacctgtccc acacctagaa gatggtgtgc cgaggcctgg tgagagatca
     4321 caaagcccaa taagagcggc ttcatctgag gcccctgaac ccctatcatg gggagctgga
     4381 aaggctggag ggtggcctgt aggaggtggc ctcgggaacc attctggtgg atgggtgccc
     4441 caattcctca cgagatcaga agaacctgaa gacagtgtcc acagatcccc ctgccatgca
     4501 ggagactgcc agctaaatgg accgaccctg agccatatgg acagctggga taacagagat
     4561 aattcaagcc aactccagcc agggagccat tcatcgtgca gccaatgtgg gaaaacatac
     4621 tgccaatctg ggagcctcct gaaccataac acgaataaaa cggacagaca ttactgcctt
     4681 ttgtgttcaa aagaattttt aaaccctgta gcgacaaaaa gccacagcca taaccatata
     4741 gatgcccaga cttttgcctg ccctgactgc gggaaggctt ttgaaagcca ccaagagcta
     4801 gcgagccatt tacaggccca cgcgaggggc cacagccaag tgccagcaca gatggaagag
     4861 gcaagggacc caaaagctgg aactggagaa gaccaagttg ttttacctgg ccaaggaaag
     4921 gcccaagagg caccatctga gacaccgaga ggaccaggtg aatctgtaga gagggcaaga
     4981 ggcggccagg ctgtgacatc gatggcggct gaagataaag agaggccatt caggtgtacc
     5041 cagtgtggga gatcatacag acatgctgga tccctattaa accaccaaaa agcacacact
     5101 actggtttat acccgtgttc cctctgccca aaactcctgc cgaacctcct ttcattaaaa
     5161 aaccattcaa ggacacacac tgacccaaag agacactgct gttcaatatg cggaaaggca
     5221 ttcagaacgg ctgcaagatt agaaggccat ggcagagtcc atgccccgag ggaaggccct
     5281 tttacgtgcc cccactgccc gagacatttc agaaggagga tttcttttgt ccaacaccaa
     5341 caacaacacc aagaagagtg gactgtagct ggatcaggga gaggccatga aggttcccaa
     5401 gaagaagtcg gaacccagtg gagaggaaag agcagcccaa aagtaggtgg aggtgccaga
     5461 tcagagagga gagaaccaag aggtttcaac tttcttgtac aaagttggca ttataagaaa
     5521 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     5581 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     5641 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     5701 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     5761 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     5821 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     5881 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     5941 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     6001 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     6061 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     6121 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     6181 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     6241 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     6301 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     6361 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     6421 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     6481 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     6541 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     6601 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     6661 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6841 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7321 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7381 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7441 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7501 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7561 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
     7621 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     7681 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
     7741 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     7801 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
     7861 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
     7921 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     7981 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttg
//
