LOCUS       sequence-191730-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression construct to express HA-tagged CDC34.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Feng Q, Jagannathan S, Bradley RK
  TITLE     The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 
            Ubiquitin Ligase Activity.
  JOURNAL   Mol Cell. 2017 Jul 20;67(2):239-251.e6. doi: 
            10.1016/j.molcel.2017.05.034. Epub 2017 Jun 29.
  PUBMED    28669802
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.05.034"; journalName: "Mol Cell"; date: 
            "2017-07-20- 20"; volume: "67"; issue: "2"; pages: "239-251.e6"
COMMENT     Sequence Label: pUB6-CDC34-HA (pRKB261)
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(135..176)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaactcaatg gtgatggtga
      121 tgatgaccgg tacgcgtaga atcgagaccg aggagagggt tagggatagg cttaccttcg
      181 aagggccctc tagactcgag ggccgaggcg gccgacatgt tttttttttt tttttttttt
      241 ttttttttcg taaagtagtt ttatttagat ttcaaacaaa ccaaagcagg gaaaagagtc
      301 ggccacggtg aatccgtcca ctctgtgctg gacgtggctc caggacctgg agctgacagg
      361 caggaccggg cccctcggac cgctacacct gggcctccca ggctggtagt gtcaggaaac
      421 ggccccccgc ccacgttccc agcagcgccc ccgtggctcc tccggggtgc ggccagtccg
      481 gaagctgggg gaccccggta gaagtcgggc tcagctcccc tcccgagggg acaggtgggc
      541 cggccgctcc caccctgggc ccgtccaccg agccccgagt gacgtgagtg gcggtggggc
      601 agcccctctt ctctgaagca cgtgaaaacc cagaacaga
//
