LOCUS       sequence-191731-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression construct to express HA-tagged CDC34.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Feng Q, Jagannathan S, Bradley RK
  TITLE     The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 
            Ubiquitin Ligase Activity.
  JOURNAL   Mol Cell. 2017 Jul 20;67(2):239-251.e6. doi: 
            10.1016/j.molcel.2017.05.034. Epub 2017 Jun 29.
  PUBMED    28669802
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.05.034"; journalName: "Mol Cell"; date: 
            "2017-07-20- 20"; volume: "67"; issue: "2"; pages: "239-251.e6"
COMMENT     Sequence Label: pUB6-CDC34-HA (pRKB261)
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
       61 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      121 acgaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc ggccattacg
      181 gccggggaga gctgctggag cgctcggggt ccccgggcgg cggcggcggc gcagaggagg
      241 aggcaggcgg cggccccggt ggctcccccc cggacggtgc gcggcccggc ccgtctcgcg
      301 aactcgcggt ggtcgcgcgg ccccgcgctg ctccgacccc gggcccctcc gccgccgcca
      361 tggctcggcc gctagtgccc agctcgcaga aggcgctgct gctggagctc aaggggctgc
      421 aggaagagcc ggtcgaggga ttccgcgtga cactggtgga cgagggcgat ctatacaact
      481 gggaggtggc catcttcggg ccccccaaca cctactacga gggcggctac ttcaaggcgc
      541 gcctcaagtt ccccatcgac tacccatact ctccaccagc ctttcggttc ctgaccaaga
      601 tgtggcaccc taacatctac gagacggggg acgtgtgtat ctccatcctc cacccgccgg
      661 tggacgaccc ccagagcggg gagctgccct cagagaggtg gaaccccacg cagaacgtca
      721 ggaccattct cctg
//
