LOCUS       sequence-191765-        9865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GSK3A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: GSK3A_pLX307
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2776..2800)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2808..2849
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2900..3488
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2953..2973)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3371..3382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3560..3793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3871..3992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3908..3927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3962..3981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4032..4167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4187..4206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4188..4206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4216..4233)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4216..4232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4225..4247)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4374..4829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4461..4480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4671..4692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4855..4959
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4960..5028,5029..5820)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5178..5197)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5991..6579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6480..6499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6733..6750
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6867..6888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6903..6908,6909..6926,6927..6933)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6941..6957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6946..6968
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6965..6981
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6965..6981
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7000..7020
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7002..7020
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7048..7274
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7275..7455
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7502..7627
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8120..8353
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8538..8582
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8813..9023
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8835..9031
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8882..9017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9063..9665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9063..9082)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9559..9579
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9708..9825
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagcaggct
     1321 tcaccatgag cggcggcggg ccttcgggag gcggccctgg gggctcgggc agggcgcgga
     1381 ctagctcgtt cgcggagccc ggcggcggag gcggaggagg cggcggcggc cccggaggct
     1441 cggcctccgg cccaggcggc accggcggcg gaaaggcatc tgtcggggcc atgggtgggg
     1501 gcgtcggggc ctcgagctcc gggggtggac ccggcggcag cggcggagga ggcagcggag
     1561 gccccggcgc aggcactagc ttcccgccgc ccggggtgaa gctgggccgt gacagcggga
     1621 aggtgaccac agtcgtagcc actctaggcc aaggcccaga gcgctcccaa gaagtggctt
     1681 acacggacat caaagtgatt ggcaatggct catttggggt cgtgtaccag gcacggctgg
     1741 cagagaccag ggaactagtc gccatcaaga aggttctcca ggacaagagg ttcaagaacc
     1801 gagagctgca gatcatgcgt aagctggacc actgcaatat tgtgaggctg agatactttt
     1861 tctactccag tggcgagaag aaagacgagc tttacctaaa tctggtgctg gaatatgtgc
     1921 ccgagacagt gtaccgggtg gcccgccact tcaccaaggc caagttgacc atccctatcc
     1981 tctatgtcaa ggtgtacatg taccagctct tccgcagctt ggcctacatc cactcccagg
     2041 gcgtgtgtca ccgcgacatc aagccccaga acctgctggt ggaccctgac actgctgtcc
     2101 tcaagctctg cgattttggc agtgcaaagc agttggtccg aggggagccc aatgtctcct
     2161 acatctgttc tcgctactac cgggccccag agctcatctt tggagccact gattacacct
     2221 catccatcga tgtttggtca gctggctgtg tactggcaga gctcctcttg ggccagccca
     2281 tcttccctgg ggacagtggg gtggaccagc tggtggagat catcaaggtg ctgggaacac
     2341 caacccggga acaaatccga gagatgaacc ccaactacac ggagttcaag ttccctcaga
     2401 ttaaagctca cccctggaca aaggtgttca aatctcgaac gccgccagag gccatcgcgc
     2461 tctgctctag cctgctggag tacaccccat cctcaaggct ctccccacta gaggcctgtg
     2521 cgcacagctt ctttgatgaa ctgcgatgtc tgggaaccca gctgcctaac aaccgcccac
     2581 ttccccctct cttcaacttc agtgctggtg aactctccat ccaaccgtct ctcaacgcca
     2641 ttctcatccc tcctcacttg aggtccccag cgggcactac caccctcacc ccgtcctcac
     2701 aagctttaac tgagactccg accagctcag actggcagtc gaccgatgcc acacctaccc
     2761 tcactaactc ctccgaccca gctttcttgt acaaagtggt tgatatcggt aagcctatcc
     2821 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtaaccac ccggtggcgc
     2881 atggacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2941 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3001 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3061 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3121 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3181 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3241 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3301 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3361 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3421 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3481 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3541 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3601 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3661 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3721 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3781 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3841 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3901 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3961 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4021 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4081 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4141 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4201 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4261 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4321 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4381 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4441 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4501 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4561 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4621 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4681 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4741 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4801 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4861 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4921 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4981 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5041 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5101 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5161 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5221 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5281 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5341 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5401 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5461 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5521 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5581 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5641 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5701 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5761 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5821 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5881 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6121 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6601 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6661 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6721 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6781 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6841 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6901 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6961 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7021 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7081 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7141 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7201 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7261 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7321 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7381 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7441 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7501 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7561 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7621 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7681 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7741 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7801 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7861 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7921 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7981 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8041 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8101 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8161 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8221 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8281 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8341 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8401 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8461 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8521 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8581 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8641 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8701 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     8761 attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8821 aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8881 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8941 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9001 aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta
     9061 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     9121 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     9181 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     9241 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
     9301 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     9361 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     9421 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     9481 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     9541 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     9601 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
     9661 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     9721 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9781 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     9841 gacagcagag atccactttg gcgcc
//
