LOCUS       sequence-191779-       16025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression and isolation of recombinant At-PIP2;1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16025)
  AUTHORS   Qing D, Yang Z, Li M, Wong WS, Guo G, Liu S, Guo H, Li N
  TITLE     Quantitative and Functional Phosphoproteomic Analysis Reveals that 
            Ethylene Regulates Water Transport via the C-Terminal 
            Phosphorylation of Aquaporin PIP2;1 in Arabidopsis.
  JOURNAL   Mol Plant. 2016 Jan 4;9(1):158-74. doi: 10.1016/j.molp.2015.10.001. 
            Epub 2015 Oct 22.
  PUBMED    26476206
REFERENCE   2  (bases 1 to 16025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2015.10.001"; journalName: "Mol Plant"; date: 
            "2016-01-4- 4"; volume: "9"; issue: "1"; pages: "158-74"
COMMENT     Sequence Label: pHub10-His-YFP-PIP2;1
FEATURES             Location/Qualifiers
     source          1..16025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        782..1127
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1188..1211
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             join(1212..1214,1215..1217,1218..1928)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1257..1278)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1518..1537)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1865..1886
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3503..3755
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3764..3781)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3764..3780)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3773..3795)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3920..3939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4130..4151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    4398..4422
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6018..7166)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7465..8259)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  8451..9218
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      9841..10552
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(10679..11050)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11083..11192
                     /note="incP origin of transfer"
     primer_bind     11415..11434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11668..11685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(11873..12523)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    12988..13012
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        13127..13803
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             13870..14895
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAV*TDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    14936..15110
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    15408..15429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15444..15449,15450..15467,15468..15474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15482..15498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15487..15509
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15506..15522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15506..15522
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
       61 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      121 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      181 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      241 gtggattgat gtgatatctc cactgacgta agggatgacg cactgcaggt ccccagatta
      301 gccttttcaa tttcagaaag aatgctaacc cacagatggt tagagaggct tacgcagcag
      361 gtctcatcaa gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga
      421 aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata
      481 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac
      541 caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca
      601 tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt
      661 tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc
      721 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
      781 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
      841 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
      901 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
      961 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     1021 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     1081 aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactctag
     1141 aggatcctca gtcacacaaa gagtaaagaa gaacaatggt cgacatgcat catcatcacc
     1201 accatcatca tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1261 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1321 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1381 cctggcccac cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg
     1441 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1501 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1561 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1621 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1681 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1741 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1801 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1861 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1921 tgtacaagcg gcgcgccatg gcaaaggatg tggaagccgt tcccggagaa ggatttcaga
     1981 caagagacta tcaagatccg ccaccagctc cgtttattga tggagcggag ctaaagaagt
     2041 ggtctttcta cagagcagtt atcgcagagt tcgtagccac tctcctcttc ttatacatca
     2101 ccgttttgac agtcatcggt tacaagattc agtccgatac tgatgccggt ggcgtagatt
     2161 gcggcggagt tggaatcctc ggtatcgctt gggcctttgg tggtatgatc ttcatcctcg
     2221 tctactgcac cgccggtatc tctggtatga tccttcttta atcttgattt tggtttggtt
     2281 tagattaaga ttatacactt tttgagtatt agaattacaa ttaaaccgaa taagctgaat
     2341 aagttttact tatgtaagta caacttgcaa actcaaaatt tagacaagta aaaattatat
     2401 ggtatggttt tggtttgttt attcgaaatt aattattttg caaggaaaaa tattgcttgg
     2461 ttgacattac tatttaatcg gtttaaaatc ttaggtttaa aagacgaaaa tgatttgttt
     2521 ataaaacatt taataagggt catgatcaaa tcggttctta tccgaatgat atatatctaa
     2581 tctaagccta gtaattattt taagcaactg actaaccctg tcttaaccag aattgaaacc
     2641 ggttaattaa ttgcttgttg taccttaatt ctcaagtact ggtaacattg tcaactcttt
     2701 ttgtttcctt aggtggtcac attaacccag cggtgacatt tgggctattc ttggcacgta
     2761 aagtgtcgtt acctagggcc ctattgtaca taatcgctca gtgtttgggt gcgatttgtg
     2821 gagttggttt tgtcaaagcc ttccaaagct cttactacac ccgttacgga ggtggagcca
     2881 actctctagc cgatggctac agcacaggga ccggtctagc cgcagagatc attggtactt
     2941 tcgttcttgt ctacaccgtc ttctctgcca ctgaccccaa acgtagtgcc agagactccc
     3001 acgttccggt gagtcacata tgtagtgcta ttaatcttca atatggttaa ccgactcaaa
     3061 accggaacca aagcaaacca aaccaacatt tgatttcaat catactaata aatttgaatt
     3121 ttgatgaaat aaaaggtgtt ggcgccactt ccaatcggat ttgccgtgtt catggtacat
     3181 ttggctacca ttcccattac cggaaccgga attaacccgg caaggagttt cggagctgcc
     3241 gtaatctaca acaagagcaa gccatgggat gaccacgtaa gacctcaacg tttctccatt
     3301 acattcaact atatttttca tttttacaag ggaataatgt tttgaaattt tggtgattac
     3361 aaacagtgga tattttgggt tggaccattc attggagctg cgatagctgc attctaccac
     3421 caattcgttc tgagagcttc aggttctaag tctcttggat cattcagaag tgctgccaac
     3481 gtctaagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     3541 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     3601 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     3661 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     3721 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttcactggcc gtcgttttac
     3781 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3841 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3901 gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4021 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     4081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4141 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     4201 ttgccgattt cggaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     4261 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     4321 cactggtgaa aagaaaaacc accccagtac attaaaaacg tccgcaatgt gttattaagt
     4381 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     4441 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     4501 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     4561 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     4621 gttgattgta acgatgacag agcgttgctg cctgtgatca aatatcatct ccctcgcaga
     4681 gatccgaatt atcagccttc ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt
     4741 gagaactatg ccgacataat aggaaatcgc tggataaagc cgctgaggaa gctgagtggc
     4801 gctatttctt tagaagtgaa cgttgacgat atcaactccc ctatccattg ctcaccgaat
     4861 ggtacaggtc ggggacccga agttccgact gtcggcctga tgcatccccg gctgatcgac
     4921 cccagatctg gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat
     4981 cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc
     5041 gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac
     5101 gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg
     5161 ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc
     5221 tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc
     5281 agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc
     5341 agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg
     5401 caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag
     5461 gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa
     5521 taaacccgcc ggcaacgccc gcagcagcat accggcgacc cctcggcctc gctgttcggg
     5581 ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt
     5641 gaagaccgac agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg
     5701 caaccgttca gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa
     5761 catctctgga gctttcttca gggccgacaa tcggatctcg cggaaatcct gcacgtcggc
     5821 cgctccaagc cgtcgaatct gagccttaat cacaattgtc aattttaatc ctctgtttat
     5881 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
     5941 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
     6001 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     6061 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     6121 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     6181 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     6241 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     6301 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     6361 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     6421 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     6481 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     6541 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     6601 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     6661 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     6721 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     6781 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
     6841 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     6901 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     6961 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     7021 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     7081 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     7141 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
     7201 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
     7261 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
     7321 ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc
     7381 tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg
     7441 gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat
     7501 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
     7561 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
     7621 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
     7681 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
     7741 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
     7801 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
     7861 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
     7921 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
     7981 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
     8041 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
     8101 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
     8161 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
     8221 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
     8281 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
     8341 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
     8401 ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg
     8461 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     8521 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     8581 ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac
     8641 accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga
     8701 tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa
     8761 aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg
     8821 cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt
     8881 acgcgtatga cagtctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg
     8941 cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacgg
     9001 atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat
     9061 atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca cggctgggac
     9121 ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc gggcattatc
     9181 tgaacataaa acactatcaa taagttggag tcattaccca attatgatag aatttacaag
     9241 ctataaggtt attgtcctgg gtttcaagca ttagtccatg caagttttta tgctttgccc
     9301 attctataga tatattgata agcgcgctgc ctatgccttg ccccctgaaa tccttacata
     9361 cggcgatatc ttctatataa aagatatatt atcttatcag tattgtcaat atattcaagg
     9421 caatctgcct cctcatcctc ttcatcctct tcgtcttggt agctttttaa atatggcgct
     9481 tcatagagta attctgtaaa ggtccaattc tcgttttcat acctcggtat aatcttacct
     9541 atcacctcaa atggttcgct gggtttatcg cacccccgaa cacgagcacg gcacccgcga
     9601 ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg
     9661 ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc gccgccctca ctgcccggca
     9721 cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc
     9781 tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg actgccagcg
     9841 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
     9901 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
     9961 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
    10021 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
    10081 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
    10141 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
    10201 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
    10261 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
    10321 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
    10381 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
    10441 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
    10501 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcggtctt
    10561 gccttgctcg tcggtgatgt acttcaccag ctccgcgaag tcgctcttct tgatggagcg
    10621 catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc
    10681 atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct
    10741 cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt
    10801 cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg
    10861 ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg
    10921 ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc
    10981 gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt
    11041 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
    11101 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
    11161 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
    11221 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
    11281 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagcgc cacgcttccc
    11341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    11401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    11461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11581 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11641 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11701 cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt ggacgctagg gcagggcatg
    11761 aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt ggaaaggggg aggggatgtt
    11821 gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct gatcaatcgt
    11881 caccctttct cggtccttca acgttcctga caacgagcct ccttttcgcc aatccatcga
    11941 caatcaccgc gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta
    12001 tcgcggcccg caacagcggc gagagcggag cctgttcaac ggtgccgccg cgctcgccgg
    12061 catcgctgtc gccggcctgc tcctcaagca cggccccaac agtgaagtag ctgattgtca
    12121 tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg
    12181 cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat
    12241 cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc
    12301 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca
    12361 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc
    12421 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt
    12481 ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac actttatcac
    12541 tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcg ttcaatcgga
    12601 ccagcggagg ctggtccgga ggccagacgt gaaacccaac atacccctga tcgtaattct
    12661 gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc tgccgggcct
    12721 cctgcgcgat ctggttcact cgaacgacgt caccgcccac tatggcattc tgctggcgct
    12781 gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg
    12841 gcggcggcca atcttgctcg tctcgctggc cggcgccaga tctggggaac cctgtggttg
    12901 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
    12961 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
    13021 tagtttaaac gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct
    13081 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
    13141 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
    13201 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
    13261 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
    13321 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac
    13381 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
    13441 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
    13501 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
    13561 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
    13621 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
    13681 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
    13741 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
    13801 tgaaatcacc agtctctctc tacaaatcta tctctctcga gctttcgcag atccgggggg
    13861 caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
    13921 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
    13981 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
    14041 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
    14101 cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg ttcacagggt
    14161 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag
    14221 gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
    14281 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
    14341 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
    14401 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
    14461 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
    14521 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
    14581 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
    14641 ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
    14701 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
    14761 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctag
    14821 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
    14881 agggcaaaga aatagagtag atgccgaccg ggatctgtcg atcgacaagc tcgagtttct
    14941 ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct
    15001 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
    15061 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta
    15121 atcgatggtt tcattggtga cgtttccggc cttgctaatg gtaatggtgc tactggtgat
    15181 tttgctggct ctaattccca aatggctcaa gtcggtgacg gtgataattc acctttaatg
    15241 aataatttcc gtcaatattt accttccctc cctcaatcgg ttgaatgtcg cccttttgtc
    15301 tttggcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    15361 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    15421 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    15481 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
    15541 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
    15601 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
    15661 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
    15721 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
    15781 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
    15841 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
    15901 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
    15961 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
    16021 gggca
//
