LOCUS       sequence-191800-       10423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary construct of cytosolic sfCFP1-10 tag for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Park E, Lee HY, Woo J, Choi D, Dinesh-Kumar SP
  TITLE     Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type
            III Secretion Using Split Fluorescent Protein Fragments.
  JOURNAL   Plant Cell. 2017 Jul;29(7):1571-1584. doi: 10.1105/tpc.17.00047. 
            Epub 2017 Jun 14.
  PUBMED    28619883
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00047"; journalName: "Plant Cell"; date: "2017-07"; 
            volume: "29"; issue: "7"; pages: "1571-1584"
COMMENT     Sequence Label: SPDK3184
FEATURES             Location/Qualifiers
     source          1..10423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     476..498
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     490..507
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     491..507
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1234..1279
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             1309..1950
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      1990..2242
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2259..2275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2259..2275)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2272..2294)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2283..2299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2307..2313,2314..2331,2332..2337))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2352..2373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2548..2572
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3873..4502
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4931..6004
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6070..6264
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6500..6522)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6608..6748
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6663..6682
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6763..6780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6934..7522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7014..7033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7768..8559)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    9080..9104
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9182..9356
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9413..9946)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(10009..10423,1..262))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
       61 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
      121 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
      181 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
      241 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
      301 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gcattcgcca
      361 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      421 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      481 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt caacatggtg
      541 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccagagg
      601 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
      661 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat
      721 cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat
      781 ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      841 caagtggatt gatgtgatgg tcaacatggt ggagcacgac actctcgtct actccaagaa
      901 tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac aaagggtaat
      961 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
     1021 agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     1081 agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga acatcgtgga
     1141 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     1201 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     1261 ttcatttcat ttggagaggc cttctagagc ggccgcggat ccggtaccat gtccaaagga
     1321 gaagaactgt ttaccggtgt tgtgccaatt ttggttgaac tcgatggtga tgtcaacgga
     1381 cataagttct cagtgagagg cgaaggagaa ggtgacgcca ccattggaaa attgactctt
     1441 aaattcatct gtactactgg taaacttcct gtaccatggc cgactctcgt aacaacgctt
     1501 acgtggggag ttcagtgctt ttcgagatac ccagaccata tgaaaagaca tgactttttt
     1561 aagtcggcta tgcctgaagg ttacgtgcaa gaaagaacaa tttcgttcaa agatgatgga
     1621 aaatataaaa ctagagcagt tgttaaattt gaaggagata ctttggttaa ccgcattgaa
     1681 ctgaaaggaa cagattttaa agaagatggt aatattcttg gacacaaact cgaatacaat
     1741 tttaatagtc ataacgtata catcactgct gataagcaaa agaacggaat taaagcgaat
     1801 ttcacagtac gccataatgt agaagatggc agtgttcaac ttgccgacca ttaccaacaa
     1861 aacaccccta ttggagacgg tccggtactt cttcctgata atcactacct ctcaacacaa
     1921 acagtcctga gcaaagatcc aaatgaaaaa ggaacaggtg gcggcggaag ttagagctcg
     1981 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     2041 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     2101 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2161 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     2221 gtgtcatcta tgttactaga tcgggaattc gtaatcatgt catagctgtt tcctgtgtga
     2281 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2341 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2401 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2461 ggtttgcgta ttggctagag cgctctagag cagcttgagc ttggatcaga ttgtcgtttc
     2521 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     2581 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     2641 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     2701 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
     2761 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     2821 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     2881 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     2941 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     3001 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     3061 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     3121 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     3181 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     3241 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     3301 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     3361 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     3421 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     3481 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     3541 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     3601 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     3661 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     3721 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     3781 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     3841 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     3901 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     3961 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     4021 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     4081 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     4141 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     4201 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     4261 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     4321 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     4381 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     4441 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     4501 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     4561 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     4621 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     4681 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     4741 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     4801 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     4861 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
     4921 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     4981 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     5041 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     5101 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     5161 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     5221 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     5281 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     5341 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     5401 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     5461 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     5521 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     5581 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     5641 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     5701 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     5761 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     5821 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     5881 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     5941 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     6001 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     6061 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     6121 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     6181 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     6241 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
     6301 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     6361 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     6421 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     6481 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6541 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6601 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     6661 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6721 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7561 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat gatatatctc ccaatttgtg
     7621 tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg
     7681 tgagcaatta tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt
     7741 cggcttgaac gaatttctag ctagacatta tttgccgact accttggtga tctcgccttt
     7801 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     7861 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     7921 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     7981 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     8041 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     8101 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     8161 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     8221 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     8281 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     8341 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     8401 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     8461 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     8521 tagttgagtc gatacttcgg cgatcaccgc ttcccccatg atgtttaact ttgttttagg
     8581 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     8641 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata
     8701 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     8761 ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt
     8821 ccactgggtt cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     8881 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     8941 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     9001 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     9061 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     9121 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     9181 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     9241 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     9301 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
     9361 agcttgtcga tcgactctag gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     9421 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     9481 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     9541 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     9601 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
     9661 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     9721 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     9781 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     9841 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     9901 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taccatggtc gatcgacaga
     9961 tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
    10021 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
    10081 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
    10141 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
    10201 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
    10261 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
    10321 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
    10381 gactgtatct ttgatattct tggagtagac gagagtgtcg tgc
//
