LOCUS       sequence-191805-        8722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral Tet-On vector for CMV-driven rtTA with EGFP and 
            Blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA2640
FEATURES             Location/Qualifiers
     source          1..8722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(155..166)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             280..297
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        460..867
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(1609..1628)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1728..1750
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1788..1806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1874..1978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1979..2047,2048..2839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2197..2216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3010..3598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3499..3518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3752..3769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3992..4508
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5058..5074)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5059..5075)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     enhancer        5159..5538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5539..5742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5693..5713
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5739..5763
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5845..6462,6463..6468,6469..6504,6505..6507,6508..6543,
                     6544..6546,6547..6582,6583..6591)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6633..7206
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6801..6818)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7028..7047
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7209..7211,7212..7214,7215..7922)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(7209..7211,7212..7214,7215..7922)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(7254..7275)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7515..7534)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7862..7883
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      7926..7935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7932..8330
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(8406..8722,1..272)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8459..8479)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
       61 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
      121 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
      181 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
      241 gagtcggatc tccctttggg ccgcctcccc gcatcggccc accaccacca ccaccacatg
      301 tgaatgatcc aagcttatcg ataggcctag gcctatcgat aggcctaggc ctatcgatag
      361 gcctaacacg agccatagat aaaataaaag attttattta gtctccagaa aaagggggga
      421 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
      481 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
      541 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
      601 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
      661 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
      721 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
      781 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
      841 gtcctccgac agactgagtc gcccgggtac ccgtgttctc aataaaccct cttgcagttg
      901 catccgactc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
      961 tcagcggggg tctttcagta ggatctcgac cgatgccctt gagagccttc aacccagtca
     1021 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     1081 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     1141 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     1201 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     1261 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     1321 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     1381 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     1441 cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg
     1501 cctcggcgag cacatggaac gggttggcat ggattgtagg cgcctgatgc ggtattttct
     1561 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     1621 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     1681 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1741 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     1801 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1861 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1981 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2041 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2101 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2161 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2221 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2281 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2341 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2401 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2461 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2521 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2581 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2641 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2701 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2761 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2821 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagggtcga
     3841 gggggggccc gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag
     3901 ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag atagaataaa
     3961 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     4021 agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
     4081 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
     4141 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
     4201 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     4261 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     4321 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     4381 gactgagtcg cccgggtacc cgtattccca ataaagcctc ttgctgtttg catccgaatc
     4441 gtggactcgc tgatccttgg gagggtctcc tcagattgat tgactgccca cctcgggggt
     4501 ctttcatttg gaggttccac cgagatttgg agacccctgc ccagggacca ccgacccccc
     4561 cgccgggagg taagctggcc agcggtcgtt tcgtgtctgt ctctgtcttt gtgcgtgttt
     4621 gtgccggcat ctagtgtttg cgcctgcgtc tgtactagtt ggctaactag atctgtatct
     4681 ggcggtcccg cggaagaact gacgagttcg tattcccggc cgcagcccct gggagacgtc
     4741 ccagcggcct cgggggcccg ttttgtggcc cattctgtat cagttaacct acccgagtcg
     4801 gactttttgg agctccgcca ctgtccgagg ggtacgtggc tttgttgggg gacgagagac
     4861 agagacactt cccgcccccg tctgaatttt tgctttcggt tttacgccga aaccgcgccg
     4921 cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     4981 tgtctgaaaa ttagctcgac aaagttaagt aatagtccct ctctccaagc tcacttacag
     5041 gcggccatca agcttatcga taccgtcgac ctcgaggagc ttggcccatt gcatacgttg
     5101 tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga
     5161 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     5221 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     5281 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     5341 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     5401 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     5461 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     5521 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     5581 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     5641 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     5701 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     5761 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     5821 agcctccgcg gccccgaatt caccatgtct agactggaca agagcaaagt cataaacggc
     5881 gctctggaat tactcaatgg agtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     5941 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     6001 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     6061 gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca
     6121 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     6181 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     6241 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     6301 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     6361 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     6421 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt
     6481 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     6541 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
     6601 gatcccccgg gctgcaggaa ttcgatggcc gccccctctc cctccccccc cccctaacgt
     6661 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6721 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     6781 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     6841 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     6901 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     6961 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     7021 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     7081 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7141 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gtttcccttt gaaaaacacg
     7201 ataataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     7261 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     7321 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     7381 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     7441 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     7501 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     7561 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     7621 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     7681 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     7741 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     7801 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     7861 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     7921 accgtgccac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     7981 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     8041 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     8101 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     8161 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     8221 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     8281 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa ggtacaagtc
     8341 cggactcaga tccagcgctg ataacccagc ctccaccaca aacaaggatg ccggtaccgg
     8401 ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8461 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8521 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8581 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8641 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8701 tccctattgc cacggcggaa ct
//
