LOCUS       sequence-191817-        3657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea foxD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Roberts-Galbraith RH, Newmark PA
  TITLE     Follistatin antagonizes activin signaling and acts with notum to 
            direct planarian head regeneration.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1363-8. doi: 
            10.1073/pnas.1214053110. Epub 2013 Jan 7.
  PUBMED    23297191
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1214053110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-22- 22"; volume: "110"; issue: "4"; pages: "1363-8"
COMMENT     Sequence Label: pRRG159-foxD
FEATURES             Location/Qualifiers
     source          1..3657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(786..833)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        847..865
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        869..887
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     869..886
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1321..1339)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1343..1361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1375..1422
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1829..2623
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1883..1902)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2493..2512
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2641..2709,2710..3501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2859..2878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
      781 cgcgtcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc taggcgcgcc
      841 ggatcctaat acgactcact atagggagat ttaggtgaca ctatagaagt gaccttgatt
      901 tcccggttct cttggtattt ttatgaagca atcattcaaa gataaattat gacgaatact
      961 attttgccag gctggaaatc tatctttata atatggaaaa cgtcccatta taaattcaca
     1021 tattccactc aatgtcaatt ttctttgagg agatcttaaa attgccatgg taattagagc
     1081 tatatatgaa tatggaggtt ttacattatg agatttagat ttgcatcttt cttgagacat
     1141 cggtatttta ttatcaacat cttttttgtc ttctgaacat attcgatcta aatcactttc
     1201 tgagtgtata ttatcatcac tttcgcattc tttcaattcg tctacatcca catcatcttc
     1261 tcgaagtaag ttttttattt gttcaatagt ctgagaatct ttgaacgcct ggaaggtctc
     1321 cctttagtga gggttaattc tccctatagt gagtcgtatt aggatccggc gcgcctagca
     1381 taaccccttg gggcctctaa acgggtcttg aggggttttt tgacgcgtcg atttcggcct
     1441 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaattcagg
     1501 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
     1561 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
     1621 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac
     1681 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     1741 agtaaactgg atggctttct tgccgccaat gatctgatgg cgcaggggat caagatctga
     1801 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     1861 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     1921 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     1981 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     2041 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     2101 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tcccaccttg ctcctgccga
     2161 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     2221 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     2281 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     2341 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     2401 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     2461 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     2521 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     2581 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattgaaa aaggaagagt
     2641 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2701 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2761 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2821 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2881 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2941 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3001 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3061 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3121 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3181 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3241 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3301 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3361 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3421 acgacgggga gccaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3481 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3541 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3601 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaag
//
