LOCUS       sequence-19183-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf100
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             625..666
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             676..693
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tacccttcac cgaattccag ctgaccacca tggattcagg gatatacgaa acaccgataa
       61 attataaaaa aagcaatgtg tccgctgttt ccgttaataa tactattttc gtaactggcg
      121 gattattcat aaataactct aatagcacga tcgtggttaa caatatggaa aaacttgaca
      181 tttataaaga caaacaatgg tcgattatag aaatgcctat ggctagggta tatcacggca
      241 tcgactcgac atttggaatg ttatattttg ccggaggtct atccgttacc gaacaatatg
      301 gtaatttaga gaaaaacaac gagatatctt gttacaatcc tagaacgaat aagtggtttg
      361 atatttcata tactatttat aagatatcca tatcatcatt gtgtaaacta aataacgtct
      421 tctatgtatt tagtaaggac attggatatg tggaaaagta tgatggtgca tggaagttag
      481 tacatgatcg tctccccgct ataaaggcat aatcaacttc tccttattga gcaattcccg
      541 gggatccgaa taaagggtca agacaattct gcagatatcc agcacagtgg cggccgctcg
      601 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
      661 tctacgcgta ccggtcatca tcaccatcac cattg
//
