LOCUS       sequence-19184-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf101
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             363..404
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             414..431
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(454..471)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    460..686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggtaccgagc tcggattcag tacccttcac cgaattccag ctgaccacca tggatacaga
       61 tgttacaaat gtagaggata tcataaatga aatagataga gagaaagaag aaatactaaa
      121 aaatgtagaa attgaaaata ataaaaacat taacaagaat catccaagtg gatatattag
      181 agaagcactc gttattaata ccagtagtaa tagtgattcc attgataaag aagttataga
      241 atgtatctgt cacgatgtag gaatataggc aattcccggg gatccgaata aagggtcaag
      301 acaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
      361 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
      421 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
      481 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      541 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      601 tgggggggtg ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      661 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
      721 ggggtatccc cacgc
//
