LOCUS       sequence-191867-       11983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TET1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Jin C, Lu Y, Jelinek J, Liang S, Estecio MR, Barton MC, Issa JP
  TITLE     TET1 is a maintenance DNA demethylase that prevents methylation 
            spreading in differentiated cells.
  JOURNAL   Nucleic Acids Res. 2014 Jun;42(11):6956-71. doi: 10.1093/nar/gku372.
            Epub 2014 May 29.
  PUBMED    24875481
REFERENCE   2  (bases 1 to 11983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku372"; journalName: "Nucleic Acids Res"; date: 
            "2014-06"; volume: "42"; issue: "11"; pages: "6956-71"
COMMENT     Sequence Label: pIRES-hrGFP II-TET1
FEATURES             Location/Qualifiers
     source          1..11983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     496..515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        740..1119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1120..1323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1368..1387
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1368..1386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7930..8001
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     misc_feature    8037..8609
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8204..8221)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8431..8450
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8615..9334
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     primer_bind     complement(9365..9385)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9365..9383)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9403..9420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9409..9520
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(9629..10084)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9766..9787)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9978..9997
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10111..10215
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        10217..10408
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10240..10260)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10405..10539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10588..11382
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10588..11382
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10642..10661)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11252..11271
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11570..11589)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11614..11661
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
       61 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      661 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccaggcgcg ccgcgatgta
      721 cgggccagat ttacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      781 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      841 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      901 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      961 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     1021 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1081 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1141 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1201 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1261 tccgccccat tgaagcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1321 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     1381 tatagggaga cccaagcttc tggaggcccg ggctttcagg gtaccgaaga aggatccaag
     1441 gaggaattcg cccttattat ggactctgta gctatgtctc gatcccgcca tgcaaggcct
     1501 tccagattag tcaggaagga agatgtaaac aaaaaaaaga aaaacagcca actacgaaag
     1561 acaaccaagg gagccaacaa aaatgtggca tcagtcaaga ctttaagccc tggaaaatta
     1621 aagcaattaa ttcaagaaag agatgttaag aaaaaaacag aacctaaacc acccgtgcca
     1681 gtcagaagcc ttctgacaag agctggagca gcacgcatga atttggatag gactgaggtt
     1741 ctttttcaga acccagagtc cttaacctgc aatgggttta caatggcgct acgaagcacc
     1801 tctcttagca ggcgactctc ccaaccccca ctggtcgtag ccaaatccaa aaaggttcca
     1861 ctttctaagg gtttagaaaa gcaacatgat tgtgattata agatactccc tgctttggga
     1921 gtaaagcact cagaaaatga ttcggttcca atgcaagaca cccaagtcct tcctgatata
     1981 gagactctaa ttggtgtaca aaatccctct ttacttaaag gtaagagcca agagacaact
     2041 cagttttggt cccaaagagt tgaggattcc aagatcaata tccctaccca cagtggccct
     2101 gcagctgaga tccttcctgg gccactggaa gggacacgct gtggtgaagg actattctct
     2161 gaagagacat tgaatgatac cagtggttcc ccaaaaatgt ttgctcagga cacagtgtgt
     2221 gctccttttc cccaaagagc aacccccaaa gttacctctc aaggaaaccc cagcattcag
     2281 ttagaagagt tgggttcacg agtagaatct cttaagttat ctgattctta cctggatccc
     2341 attaaaagtg aacatgattg ctaccccacc tccagtctta ataaggttat acctgacttg
     2401 aaccttagaa actgcttggc tcttggtggg tctacgtctc ctacctctgt aataaaattc
     2461 ctcttggcag gctcaaaaca agcgaccctt ggtgctaaac cagatcatca agaggccttc
     2521 gaagctactg caaatcaaca ggaagtttct gataccacct ctttcctagg acaggccttt
     2581 ggtgctatcc cacatcaatg ggaacttcct ggtgctgacc cagttcatgg tgaggccctg
     2641 ggtgagaccc cagatctacc agagattcct ggtgctattc cagtccaagg agaggtcttt
     2701 ggtactattt tagaccaaca agaaactctt ggtatgagtg ggagtgttgt cccagacttg
     2761 cctgtcttcc ttcctgttcc tccaaatcca attgctacct ttaatgctcc ttccaaatgg
     2821 cctgagcccc aaagcactgt ctcatatgga cttgcagtcc agggtgctat acagattttg
     2881 cctttgggct caggacacac tcctcaatca tcatcaaact cagagaaaaa ttcattacct
     2941 ccagtaatgg ctataagcaa tgtagaaaat gagaagcagg ttcatataag cttcctgcca
     3001 gctaacactc aggggttccc attagcccct gagagaggac tcttccatgc ttcactgggt
     3061 atagcccaac tctctcaggc tggtcctagc aaatcagaca gagggagctc ccaggtcagt
     3121 gtaaccagca cagttcatgt tgtcaacacc acagtggtga ctatgccagt gccaatggtc
     3181 agtacctcct cttcttccta taccactttg ctaccgactt tggaaaagaa gaaaagaaag
     3241 cgatgtgggg tctgtgaacc ctgccagcag aagaccaact gtggtgaatg cacttactgc
     3301 aagaacagaa agaacagcca tcagatctgt aagaaaagaa aatgtgagga gctgaaaaag
     3361 aaaccatctg ttgttgtgcc tctggaggtt ataaaggaaa acaagaggcc ccagagggaa
     3421 aagaagccca aagttttaaa ggcagatttt gacaacaaac cagtaaatgg ccccaagtca
     3481 gaatccatgg actacagtag atgtggtcat ggggaagaac aaaaattgga attgaaccca
     3541 catactgttg aaaatgtaac taaaaatgaa gacagcatga caggcatcga ggtggagaag
     3601 tggacacaaa acaagaaatc acagttaact gatcacgtga aaggagattt tagtgctaat
     3661 gtcccagaag ctgaaaaatc gaaaaactct gaagttgaca agaaacgaac caaatctcca
     3721 aaattgtttg tacaaaccgt aagaaatggc attaaacatg tacactgttt accagctgaa
     3781 acaaatgttt catttaaaaa attcaatatt gaagaattcg gcaagacatt ggaaaacaat
     3841 tcttataaat tcctaaaaga cactgcaaac cataaaaacg ctatgagctc tgttgctact
     3901 gatatgagtt gtgatcatct caaggggaga agtaacgttt tagtattcca gcaacctggc
     3961 tttaactgca gttccattcc acattcttca cactccatca taaatcatca tgctagtata
     4021 cacaatgaag gtgatcaacc aaaaactcct gagaatatac caagtaaaga accaaaagat
     4081 ggatctcccg ttcaaccaag tctcttatcg ttaatgaaag ataggagatt aacattggag
     4141 caagtggtag ccatagaggc cctgactcaa ctctcagaag ccccatcaga gaattcctcc
     4201 ccatcaaagt cagagaagga tgaggaatca gagcagagaa cagccagttt gcttaatagc
     4261 tgcaaagcta tcctctacac tgtaagaaaa gacctccaag acccaaactt acagggagag
     4321 ccaccaaaac ttaatcactg tccatctttg gaaaaacaaa gttcatgcaa cacggtggtt
     4381 ttcaatgggc aaactactac cctttccaac tcacatatca actcagctac taaccaagca
     4441 tccacaaagt cacatgaata ttcaaaagtc acaaattcat tatctctttt tataccaaaa
     4501 tcaaattcat ccaagattga caccaataaa agtattgctc aagggataat tactcttgac
     4561 aattgttcca atgatttgca tcagttgcca ccaagaaata atgaagtgga gtattgcaac
     4621 cagttactgg acagcagcaa aaaattggac tcagatgatc tatcatgtca ggatgcaacc
     4681 catacccaaa ttgaggaaga tgttgcaaca cagttgacac aacttgcttc gataattaag
     4741 atcaattata taaaaccaga ggacaaaaaa gttgaaagta caccaacaag ccttgtcaca
     4801 tgtaatgtac agcaaaaata caatcaggag aagggcacaa tgcaacagaa accaccttca
     4861 agtgtacaca ataatcatgg ttcatcatta acaaaacaaa agaacccaac ccagaaaaag
     4921 acaaaatcca ccccatcaag agatcggcgg aaaaagaagc ccacagttgt aagttatcaa
     4981 gaaaatgatc ggcagaagtg ggaaaagttg tcctatatgt atggcacaat atgcgacatt
     5041 tggatagcat cgaaatttca aaattttggg caattttgtc cacatgattt tcctactgta
     5101 tttgggaaaa tttcttcctc gaccaaaata tggaaaccac tggctcaaac gaggtccatt
     5161 atgcaaccca aaacagtatt tccaccactc actcagataa aattacagag atatcctgaa
     5221 tcagcagagg aaaaggtgaa ggttgaacca ttggattcac tcagcttatt tcatcttaaa
     5281 acggaatcca acgggaaggc attcactgat aaagcttata attctcaggt acagttaacg
     5341 gtgaatgcca atcagaaagc ccatcctttg acccagccct cctctccacc taaccagtgt
     5401 gctaacgtga tggcaggcga tgaccaaata cggtttcagc aggttgttaa ggagcaactc
     5461 atgcatcaga gactgccaac attgcctggt atctctcatg aaacaccctt accggagtca
     5521 gcactaactc tcaggaatgt aaatgtagtg tgttcaggtg gaattacagt ggtttctacc
     5581 aaaagtgaag aggaagtctg ttcatccagt tttggaacat cagaattttc cacagtggac
     5641 agtgcacaga aaaattttaa tgattatgcc atgaacttct ttactaaccc tacaaaaaac
     5701 ctagtgtcta taactaaaga ttctgaactg cccacctgca gctgtcttga tcgagttata
     5761 caaaaagaca aaggcccata ttatacacac cttggggcag gaccaagtgt tgctgctgtc
     5821 agggaaatca tggagaatag gtatggtcaa aaaggaaacg caataaggat agaaatagta
     5881 gtgtacaccg gtaaagaagg gaaaagctct catgggtgtc caattgctaa gtgggtttta
     5941 agaagaagca gtgatgaaga aaaagttctt tgtttggtcc ggcagcgtac aggccaccac
     6001 tgtccaactg ctgtgatggt ggtgctcatc atggtgtggg atggcatccc tcttccaatg
     6061 gccgaccggc tatacacaga gctcacagag aatctaaagt catacaatgg gcaccctacc
     6121 gacagaagat gcaccctcaa tgaaaatcgt acctgtacat gtcaaggaat tgatccagag
     6181 acttgtggag cttcattctc ttttggctgt tcatggagta tgtactttaa tggctgtaag
     6241 tttggtagaa gcccaagccc cagaagattt agaattgatc caagctctcc cttacatgaa
     6301 aaaaaccttg aagataactt acagagtttg gctacacgat tagctccaat ttataagcag
     6361 tatgctccag tagcttacca aaatcaggtg gaatatgaaa atgttgcccg agaatgtcgg
     6421 cttggcagca aggaaggtcg tcccttctct ggggtcactg cttgcctgga cttctgtgct
     6481 catccccaca gggacattca caacatgaat aatggaagca ctgtggtttg taccttaact
     6541 cgagaagata accgctcttt gggtgttatt cctcaagatg agcagctcca tgtgctacct
     6601 ctttataagc tttcagacac agatgagttt ggctccaagg aaggaatgga agccaagatc
     6661 aaatctgggg ccatcgaggt cctggcaccc cgccgcaaaa aaagaacgtg tttcactcag
     6721 cctgttcccc gttctggaaa gaagagggct gcgatgatga cggaggttct tgcacataag
     6781 ataagggcag tggaaaagaa acctattccc cgaatcaagc ggaagaataa ctcaacaaca
     6841 acaaacaaca gtaagccttc gtcactgcca accttaggga gtaacactga gaccgtgcaa
     6901 cctgaagtaa aaagtgaaac cgaaccccat tttatcttaa aaagttcaga caacactaaa
     6961 acttattcgc tgatgccatc cgctcctcac ccagtgaaag aggcatctcc aggcttctcc
     7021 tggtccccga agactgcttc agccacacca gctccactga agaatgacgc aacagcctca
     7081 tgcgggtttt cagaaagaag cagcactccc cactgtacga tgccttcggg aagactcagt
     7141 ggtgccaatg cagctgctgc tgatggccct ggcatttcac agcttggcga agtggctcct
     7201 ctccccaccc tgtctgctcc tgtgatggag cccctcatta attctgagcc ttccactggt
     7261 gtgactgagc cgctaacgcc tcatcagcca aaccaccagc cctccttcct cacctctcct
     7321 caagaccttg cctcttctcc aatggaagaa gatgagcagc attctgaagc agatgagcct
     7381 ccatcagacg aacccctatc tgatgacccc ctgtcacctg ctgaggagaa attgccccac
     7441 attgatgagt attggtcaga cagtgagcac atctttttgg atgcaaatat tggtggggtg
     7501 gccatcgcac ctgctcacgg ctcggttttg attgagtgtg cccggcggga gctgcacgct
     7561 accactcctg ttgagcaccc caaccgtaat catccaaccc gcctctccct tgtcttttac
     7621 cagcacaaaa acctaaataa gccccaacat ggttttgaac taaacaagat taagtttgag
     7681 gctaaagaag ctaagaataa gaaaatgaag gcctcagagc aaaaagacca ggcagctaat
     7741 gaaggtccag aacagtcctc tgaagtaaat gaattgaacc aaattccttc tcataaagca
     7801 ttaacattaa cccatgacaa tgttgtcacc gtgtcccctt atgctctcac acacgttgcg
     7861 gggccctata accattgggt ctgtaggata agggcgaatt ctgcagatat ccatcacact
     7921 ggcggccgcg actacaagga tgacgatgac aaggattaca aagacgacga tgataaggac
     7981 tataaggatg atgacgacaa ataatagcaa ttcctcgacg actgcatagg gttacccccc
     8041 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     8101 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     8161 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     8221 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     8281 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     8341 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     8401 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     8461 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     8521 tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg
     8581 tggttttcct ttgaaaaaca cgatgataat gaccatggtg agcaagcaga tcctgaagaa
     8641 caccggcctg caggagatca tgagcttcaa ggtgaacctg gagggcgtgg tgaacaacca
     8701 cgtgttcacc atggagggct gcggcaaggg caacatcctg ttcggcaacc agctggtgca
     8761 gatccgcgtg accaagggcg cccccctgcc cttcgccttc gacatcctga gccccgcctt
     8821 ccagtacggc aaccgcacct tcaccaagta ccccgaggac atcagcgact tcttcatcca
     8881 gagcttcccc gccggcttcg tgtacgagcg caccctgcgc tacgaggacg gcggcctggt
     8941 ggagatccgc agcgacatca acctgatcga ggggatgttc gtgtaccgcg tggagtacaa
     9001 gggccgcaac ttccccaacg acggccccgt gatgaagaag accatcaccg gcctgcagcc
     9061 cagcttcgag gtggtgtaca tgaacgacgg cgtgctggtg ggccaggtga tcctggtgta
     9121 ccgcctgaac agcggcaagt tctacagctg ccacatgcgc accctgatga agagcaaggg
     9181 cgtggtgaag gacttccccg agtaccactt catccagcac cgcctggaga agacctacgt
     9241 ggaggacggc ggcttcgtag agcagcacga gaccgccatc gcccagctga ccagcctggg
     9301 caagcccctg ggcagcctgc acgagtgggt gtaagctcga gcatgcatct agagggccct
     9361 attcccttta gtgagggtta attgctagag ctcgctgatc agcctcgact gtgccttcta
     9421 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     9481 ctctcactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     9541 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     9601 gcaggcatgc tggggcgccg gcgcgcgtaa attgtaagcg ttaatatttt gttaaaattc
     9661 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     9721 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     9781 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     9841 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     9901 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     9961 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
    10021 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
    10081 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    10141 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    10201 tgaaaaagga agaatcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
    10261 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    10321 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10381 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccagttcc gcccattctc
    10441 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    10501 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
    10561 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
    10621 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
    10681 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
    10741 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
    10801 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
    10861 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
    10921 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
    10981 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
    11041 cttgtcgatc aggatgatct ggacgaagaa catcaggggc tcgcgccagc cgaactgttc
    11101 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
    11161 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
    11221 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagaa
    11281 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
    11341 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
    11401 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
    11461 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    11521 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
    11581 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
    11641 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
    11701 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
    11761 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
    11821 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
    11881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa agg
//
