LOCUS       sequence-191869-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TET1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Jin C, Lu Y, Jelinek J, Liang S, Estecio MR, Barton MC, Issa JP
  TITLE     TET1 is a maintenance DNA demethylase that prevents methylation 
            spreading in differentiated cells.
  JOURNAL   Nucleic Acids Res. 2014 Jun;42(11):6956-71. doi: 10.1093/nar/gku372.
            Epub 2014 May 29.
  PUBMED    24875481
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku372"; journalName: "Nucleic Acids Res"; date: 
            "2014-06"; volume: "42"; issue: "11"; pages: "6956-71"
COMMENT     Sequence Label: pIRES-hrGFP II-TET1
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             176..895
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
ORIGIN
        1 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
       61 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag
      121 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tgaccatggt
      181 gagcaagcag atcctgaaga acaccggcct gcaggagatc atgagcttca aggtgaacct
      241 ggagggcgtg gtgaacaacc acgtgttcac catggagggc tgcggcaagg gcaacatcct
      301 gttcggcaac cagctggtgc agatccgcgt gaccaagggc gcccccctgc ccttcgcctt
      361 cgacatcctg agccccgcct tccagtacgg caaccgcacc ttcaccaagt accccgagga
      421 catcagcgac ttcttcatcc agagcttccc cgccggcttc gtgtacgagc gcaccctgcg
      481 ctacgaggac ggcggcctgg tggagatccg cagcgacatc aacctgatcg aggggatgtt
      541 cgtgtaccgc gtggagtaca agggccgcaa cttccccaac gacggccccg tgatgaagaa
      601 gaccatcacc ggcctgcagc ccagcttcga ggtggtgtac atgaacgacg gcgtgctggt
      661 gggccaggtg atcctggtgt accgcctgaa cagcggcaag ttctacagct gccacatgcg
      721 caccctgatg aagagcaagg gcgtggtgaa ggacttcccc gagtaccact tcatccagca
      781 ccgcctggag aagacctacg tggaggacgg cggcttcgta gagcagcacg agaccgccat
      841 cgcccagctg accagcctgg gcaagcccct gggcagcctg cacgagtggg tgtaagctcg
      901 agcatgcatc tagagggccc
//
