LOCUS       sequence-19187-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf104
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             591..632
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             642..659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(682..699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 taccgagctc ggatccagta cccttcaccg aattccagct gaccaccatg acggccaact
       61 ttagtaccca cgtcttttca ccacagcact gtggatgtga cagactgacc agtattgatg
      121 acgtcagaca atgtttgact gaatatattt attggtcgtc ctatgcatac cgcaacaggc
      181 aatgcgctgg acaattgtat tccacactcc tctcttttag agatgatgcg gaattagtgt
      241 tcatcgacat tcgcgagctg gtaaaaaata tgccgtggga tgatgtcaaa gattgtgcag
      301 aaatcatccg ttgttatata ccggatgagc aaaaaaccat cagagagatt tcggccatca
      361 tcggactttg tgcatatgct gctactcact ggggaggtga agaccatccc actagtaaca
      421 gtctgaacgc attgtttgtg atgcttgaga tgctcaatta cgtggattat aacatcatat
      481 tccggcgtat gattgagcaa ttcccgggga tccgagtaaa gggtcaagac aattctgcag
      541 atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta
      601 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
      661 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgtt
//
