LOCUS       sequence-191882-        3681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea Unc-9 (also
            known as h.26.12f).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Roberts-Galbraith RH, Newmark PA
  TITLE     Follistatin antagonizes activin signaling and acts with notum to 
            direct planarian head regeneration.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1363-8. doi: 
            10.1073/pnas.1214053110. Epub 2013 Jan 7.
  PUBMED    23297191
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1214053110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-22- 22"; volume: "110"; issue: "4"; pages: "1363-8"
COMMENT     Sequence Label: pRRG325-Unc-9
FEATURES             Location/Qualifiers
     source          1..3681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     508..527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     761..778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(790..837)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        851..869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        873..891
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     873..890
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1349..1367)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1371..1389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1403..1450
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1857..2651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1911..1930)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2521..2540
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2669..2737,2738..3529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2887..2906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
       61 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      781 agcacgcgtc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctaggcg
      841 cgccggatcc taatacgact cactataggg agatttaggt gacactatag aagtgacctt
      901 tacaatgcgt attgccgttg aacatgtttc ttgagaaaat attcatttta ctctggtttt
      961 ggaacgtaat aatcggctgt atcaccgctg tcagtttgtt gagttggttg aacaaattag
     1021 ctaacagaaa ggacaaagtc cgattcatcg gagaatatct taaattgatc gagttgctaa
     1081 aagaatccga agaatctttg gtaaaaaagt tcattttcga ctacttacga acggatggat
     1141 tatttatagt gcatttgata tctaccaatg tcagtgatat tgtggcagct gacatagttg
     1201 gcgaactgtt ggatttgtac cgctcccaag aaaaggacga atatgaacct ttaaactcag
     1261 tagacgagat aaatttaagt gaaaacaaat taaaaaatgt tgaaaaaata attggcttgg
     1321 aaattcccgg aaaaagctac aaggtctccc tttagtgagg gttaattctc cctatagtga
     1381 gtcgtattag gatccggcgc gcctagcata accccttggg gcctctaaac gggtcttgag
     1441 gggttttttg acgcgtcgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1501 tttaacgcga attttaacaa aattcagggc gcaagggctg ctaaaggaag cggaacacgt
     1561 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
     1621 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
     1681 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
     1741 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaatga
     1801 tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga
     1861 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     1921 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     1981 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     2041 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2101 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2161 tcctgtcatc ccaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     2221 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     2281 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     2341 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     2401 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     2461 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     2521 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     2581 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     2641 agttcttctg aattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2701 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2761 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2821 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2881 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2941 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3001 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3061 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3121 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3181 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3241 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3301 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3361 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3421 taagccctcc cgtatcgtag ttatctacac gacggggagc caggcaacta tggatgaacg
     3481 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3541 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3601 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3661 ctgagcgtca gaccccgtag a
//
