LOCUS       sequence-191885-        3519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the square shown in figure 3F of associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_square
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1096..1890
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1150..1169)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1760..1779
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2055..2073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2111..2133)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2233..2252
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3336..3358)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3347..3363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3371..3377,3378..3395,3396..3401))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3416..3437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa aaatattccg
      961 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     1021 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     1081 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     1141 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     1201 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     1261 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     1321 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     1381 gaagtgccgg ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc
     1441 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     1501 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     1561 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     1621 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     1681 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     1741 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     1801 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     1861 ttctatcgcc ttcttgacga gttcttctga accggtaata ttattgaagc atttatcagg
     1921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1981 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2041 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     2101 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2161 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     2221 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccaattg gtcgacctcg
     2281 agttaattaa cgtaacgtca gttacgtcga gcagaatact gtggaaatcc aagagcgctg
     2341 attacattcg cagttagtaa gattttatca ctgtaaagat tcctatactc aagaatttta
     2401 acctcgctgg acatagatag tgagggtctc taatgtccca aactctatcc aggatggaag
     2461 aatacaatcc cacatccggt ccgtgttagc gatgctccac ggatcgccaa tgagccactt
     2521 gtaaagccgg atcctcagtc agtaaattgg actggactat ccgagcgttg tccatgcctt
     2581 tatgcttgga acgacagcga tcatctgaat acaagtctta ctcgtgataa tttgtgcgat
     2641 ctgctctgag atcgagatga tccgctgatg agcagccctc cttagcatag ctagccctaa
     2701 gtgagcttca ggccatgaga cagccaaggg gtcagaggta ttagaggtgc gctgtacagg
     2761 gggtagtgtt cgacgaaacg ttacgcctcg tatcgacttc gagatctatg aattatgatt
     2821 gtcggagcca atcctccgat ataatgcccg ggatctgagc cctgcagcac acattctcgt
     2881 gaaccgacaa ctcttgacag tgtacacccg tccgttataa catgaacgcc cgttccatgt
     2941 ccggtgaatc cacatttacc tgtatgtgga cggtgatcga gacaaaaacg gtcaatacgc
     3001 gagcattcta gtacaagaaa gcctgcatta gtgagtgaca agtcgttgta ccggttcagt
     3061 agtaccgata gtatcgccga catgggtcgg cgaggcactc ccggccatag ctagtatttc
     3121 atccaatacc tgacttttac cagtacatgt ataactacgg ccacacagtg tataaatgtc
     3181 ggttgccgtc tgctaccaaa taacgaaagt gacgcgcgaa cattactatc gattgatctc
     3241 gaacccgatg aattcagatg aatcgatatt gattgatacc agccatatta agcgccgcgg
     3301 tccagcgtgg gcgatcgctc tagagctagc gaattcctgt gtgaaattgt tatccgctca
     3361 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3421 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3481 cgtgccagct gcattaatga atcggccaac gcgcgggga
//
