LOCUS       sequence-191887-        3572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the Drigalski spatula shown in figure 3E of associated 
            article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_drigalski
FEATURES             Location/Qualifiers
     source          1..3572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..34)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(188..776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(268..287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1078..1872
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1132..1151)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1742..1761
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2037..2055
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2093..2115)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2215..2234
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3371..3393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3382..3398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3406..3412,3413..3430,3431..3436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3451..3472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
       61 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      601 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      901 gttttaaatc aatctaaagt atatatgagt aaaaatattc cggaattgcc agctggggcg
      961 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     1021 atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg
     1081 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1141 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1201 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     1261 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     1321 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     1381 ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     1441 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     1501 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     1561 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     1621 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     1681 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     1741 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     1801 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     1861 gagttcttct gaaccggtaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2041 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     2101 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     2161 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     2221 cagagcagat tgtactgaga gtgcaccaat tggtcgacct cgagttaatt aacgtaacgt
     2281 cagttacgtc gagcagaata ctgtggaaat ccaagagcgc tgattacatt cgcagttagt
     2341 aagattttat cactgtaaag attcctatac tcaagaattt taacctcgct ggacatagat
     2401 agactctatc caggatggaa gaaggaaaaa gagcatccgg tccgtgttag cgatgcattg
     2461 ggcatcgcca atgagccact tgtaacctgg ttgcaccatc gtgcacagcc ggatcctcag
     2521 tcagtaaatt ggactggact atccgagcgg aacgacagcg atcatctgaa cgttagagga
     2581 cctcgtgata atttgtgcga tcttgtatat catcgagatg atccgctgat gagttgacgc
     2641 cacagagggt accgcagccc tccttagcat agctagccct aagtgagctt caggccaggt
     2701 attagaggtg cgctgtatcg caatagtcgt tcgacgaaac gttacgcctc cgtgtgtgtt
     2761 cgagatctat gaattatgat gtagattcgt acgtgggaga ttgtcggagc caatcctccg
     2821 atataatgcc cgggatctca atacgcgagc attctagtgg gccccagcct gcattagtga
     2881 gtgacaatgc taaactcacc ttcagtagta ccgatagtat cgttgggctt gatagttaac
     2941 cgtgtatgtg gacggtgatc gagacaaaaa cggtgtatac ttgctagcta gtatttcatc
     3001 caataccacg gtgttaccag tacatgtata actagggaat tcggcgctat aaatgtcggt
     3061 tgccgtcttc ggcgaggcac tcccggccag caccgccgga gactttacag cctgactttt
     3121 tgaagtccat tttactatcg attgatctcg aagggcgtac attcagatga atcgatattg
     3181 aataacgaaa gtgatattaa gcgccgcggt ccagcgatac agaatcccga tgaggactgt
     3241 cagatggcgg gatgttgggt aaatgtggat tcaccggaga ggcaggtttc attcatgtta
     3301 taacggacgg gttataaaac tcaagagttg tcggttcacg agtgggcgat cgctctagag
     3361 ctagcgaatt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3541 ccaacgcgcg gggagaggcg gtttgcgtat tg
//
