LOCUS       sequence-191889-        4027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the cross shown in figure 3G in the associated article.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_cross
FEATURES             Location/Qualifiers
     source          1..4027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(192..780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(272..291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1082..1876
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1136..1155)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1746..1765
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2041..2059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2097..2119)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2219..2238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3830..3852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3841..3857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3865..3871,3872..3889,3890..3895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3910..3931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
       61 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      901 tgaagtttta aatcaatcta aagtatatat gagtaaaaat attccggaat tgccagctgg
      961 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     1021 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     1081 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     1141 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     1201 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     1261 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     1321 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     1381 ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     1441 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     1501 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     1561 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     1621 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     1681 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     1741 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     1801 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     1861 tgacgagttc ttctgaaccg gtaatattat tgaagcattt atcagggtta ttgtctcatg
     1921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1981 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2041 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2101 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2161 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     2221 gcatcagagc agattgtact gagagtgcac caattggtcg acctcgagtt aattaacgta
     2281 acgtcagtta cgtcgagcag aatactgtgg aaagttcatg ttataacgga cgggtgcagt
     2341 tacgtaagat tttatcactg taaaattcct atactcaaga attttaacct cgctggacat
     2401 agatagtgag ggtctctaat gtcccaaact ctatccagga tggaagaata caatcccaca
     2461 tccggtccgt gttagcgatg ctccacggat cgccaatgag ccacttgtaa agccggatcc
     2521 tcagtcagta aattggactg gactatccga gcgttgtcca tgcctttatg cttgcgttag
     2581 aggacctatc acaagtacaa gtcttactgt atatccggac taacaccggc tctgaggttg
     2641 acgccacaga gggtaccgca gccctcctta gcatagctag ccctaagtga gcttcaggcc
     2701 atgagacagc gatactatcg gtactactga acggatgagc gtcattgtca ctcactaatg
     2761 caggctacca acgactagaa tgctcgcgta ttgaacgtgg gagattgtcg gagccaatcc
     2821 tccgatataa tgcccgggat ctgagccctg cagcacagaa cgacagcgat catctgaatt
     2881 gctcagtgta tcgtgataat ttgtgcgatc tacgcccgta tcgagatgat ccgctgatga
     2941 taaccgtgta tgtggacggt gatcgagaca aaaacggtgt atacttgcat agaactccac
     3001 aagaaattgc cacggtggcg gcaatcggtc gttgtaccgg gaattcggcg cccacgccta
     3061 agccgacatg ggtcggcgag gcactcccgg ccagcaccgc cggagacttt acagcctgac
     3121 tttttgaagt ccatttagga agacggcaac cgacatttat atacatgttt tcactagtta
     3181 tacatgtact ggtaatgacg cgctattgga tgaaatacta gctaagaatc ccgatgagga
     3241 ctgtcagatg gcgggatgtt gataccagcc atgctgcgaa atgaggcagg tattagaggt
     3301 gcgctgtatg gtgatatata ttcgacgaaa cgttacgcct ctgtacgagt tcgagatcta
     3361 tgaattatga aagggactga tcccaacgcg accgcaaacc ctgtgaaacg tcctgcccta
     3421 ttccacgaca actaaagcta agagttcaag tgcatctggt ctgccgcaat tagcctaatt
     3481 taattctgaa acttagccag ggtaaatgcc atatcccccg tacagattgc ttcatttgga
     3541 ggtacatttc tgtgatcaga tttgtccggt gctggaccgc ggcgcttaat atggcaacat
     3601 tccctcaata tcgattcatc tgaatccttc gtattcgaga tcaatcgata gtaacagtgc
     3661 caggtaccaa gacgcctttg ttcaatatga gagggcaata ccggcgtaag ccagacaggt
     3721 aaatgtggat tcaccggaat tactggtcgg tccaagagcg ctgattacat tatgtgatgt
     3781 caagagttgt cggttcacga gtgggcgatc gctctagagc tagcgaattc ctgtgtgaaa
     3841 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3901 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3961 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4021 tttgcgt
//
