LOCUS       sequence-191890-        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the bent two-helix-bundle shown in figure 3B of 
            associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_bow_360
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..48
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(86..108)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     208..227
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1350..1372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1361..1377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1385..1391,1392..1409,1410..1415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1430..1451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1568..1585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1739..2327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1819..1838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2629..3423
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2683..2702)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3293..3312
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
       61 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      121 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      181 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      241 aattggtcga cctcgagtta attaacgtat ctgctcgacg taactgacgt atactgtgga
      301 aaggctagaa cccgtccgtt ataacatgaa acgagaacta acgatagcat cggtccggtg
      361 aatccacatt taccaacctc gctggacata gatactagaa tgctcgcgta ttgaaaggaa
      421 aaagagcaac tcgtatgtcc ggtccgtgtt agcgatgcga cgaaaacgcc tccacggtcg
      481 ccaatgagcc acttgtaaca gccggatcct cagtcagtaa attcagatga tcgctgtcgt
      541 tcttgtccat gcctttatgc tttaccagta catgtataac taagtacaag tcttactgta
      601 tatcctataa atgtcggttg ccgtctttga cgccacagag ggtacttcga gatcaatcga
      661 tagtaatagc cctaagtgag cttcaggcct tcgacgaaac gttacgcctc tcgcaatagt
      721 cggatgagct tcgagatcta tgaattatga gttataccaa cgaggtatcg actgtagatt
      781 cgtacgtggg agattgtcgg agccaatcct ccgatataat gcccgggatc tgagccctgc
      841 agcacacatt cgggccccag tggctcattg ctcagtgtac tgctaaactc acccgatagg
      901 acgcccgttc catggttggg cttgatagtt aaccgtgtat gtggacggtg gctggaccgc
      961 ggcgcttaat aacttgcata gaactcaata tcgattcatc tgaatggcgg caaaggtatt
     1021 agaggtgcgc tgtacggcgc ccacgcctca tcagcggatc atctcgatgg cactccagat
     1081 cgcacaaatt atcacgatta cagcctgact tagctagtat ttcatccaat agcggccacg
     1141 atactatcgg tactactgaa caccaggcta ccattgtcac tcactaatgc aggcacaatg
     1201 tgactctatc caggatggaa gaatgaggac tgtcagttta cagtgataaa atcttaccca
     1261 tgctgaatgt aatcagcgct cttggacata tactgcatgt cgtgaaccga caactcttga
     1321 gtgggcgatc gctctagagc tagcgaattc ctgtgtgaaa ttgttatccg ctcacaattc
     1381 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1441 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1501 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1561 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1621 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1681 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1741 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1801 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1861 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1921 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1981 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2041 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2101 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2161 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2221 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2281 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2341 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2401 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2461 caatctaaag tatatatgag taaaaatatt ccggaattgc cagctggggc gccctctggt
     2521 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     2581 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     2641 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     2701 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     2761 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     2821 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     2881 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     2941 tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     3001 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     3061 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     3121 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     3181 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     3241 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     3301 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     3361 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     3421 tgaaccggta atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3481 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3541 cacctgacgt ctaagaaa
//
