LOCUS       sequence-191891-        4137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the bent four-helix-bundle shown in figure 4E of 
            associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_4hb_bent
FEATURES             Location/Qualifiers
     source          1..4137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..20)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(174..762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(254..273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1064..1858
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1118..1137)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1728..1747
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2023..2041
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2079..2101)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2201..2220
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3922..3944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3933..3949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3957..3963,3964..3981,3982..3987))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4002..4023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
       61 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      901 taaagtatat atgagtaaaa atattccgga attgccagct ggggcgccct ctggtaaggt
      961 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
     1021 gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     1081 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     1141 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     1201 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     1261 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     1321 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatccca
     1381 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     1441 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     1501 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     1561 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     1621 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     1681 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     1741 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     1801 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac
     1861 cggtaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1981 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2041 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2101 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2161 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     2221 ctgagagtgc accaattggt cgacctcgag ttaattaacg tatattggat gaaatactag
     2281 ctaatactgt ggaaaggcta gaggtctgct cgacgtaact gacgtgagaa ctaacgatag
     2341 catcggttac cagtacatgt ataactaaac ctcgctggac atagatagtc caagagcgct
     2401 gattacattg gaaaaagagc aactcgtatg ttcgagatct atgaattatg agacgaaaac
     2461 gcctccacgg actataaatg tcggttgccg tctgccggat cctcagtcag taaattggac
     2521 tggactatcc gagcgttgtc catgccttta tgcttgcgtt agaggaccta tcacaagtac
     2581 aagtcttact gtatatccgg actaacaccg gctctgaggt tgacgccaca gagggtaccg
     2641 cagccctcct tagcatagct agccctaagt gagcttcagg ccatgagaca gcctcatcag
     2701 cggatcatct cgatggatga gcgtcagggg gtagtgcgtg tgtgttaggt attagaggtg
     2761 cgctgtatag attcgtacgt gggagattgt cggagccatt catgttataa cggacgggtg
     2821 atctgagccc tgcagcacac attcgggccc cactctatcc aggatggaag aactgctaaa
     2881 ctcacccgat aggacgcccg ttctcgtgaa ccgacaactc ttgaaccgtg tatgtggacg
     2941 gtgatcgaga caaaaaggta aatgtggatt caccggatcc acaagaaatt gccacggtgg
     3001 cggcaatcgg tcgttgtacc gggaattcgg cgcccacgcc taagccgaca tgggtcggcg
     3061 aggcactccc ggccagcacc gccggagact ttacagcctg actttttgaa gtccatttag
     3121 gaccgcggcc acacagtggg gcgtacatgt tttcaccagg ctaccaaata acgaaaggta
     3181 agattttatc actgtaaata cgatacagaa tcccgatgag gactgtcaga tccggtccgt
     3241 gttagcgatg ccatgctgcg aaatgaggca ggtttcaaac atactagaat gctcgcgtat
     3301 tgaaacgtaa gtacctttgg gcgtgtacga ggctgttcag tagtaccgat agtatcgact
     3361 gatcccaacg cgaccgcaaa ccctgtgttc gagatcaatc gatagtaaga caactaaagc
     3421 taagagttca agtgcatctg ttcgacgaaa cgttacgcct cttaattctg aaacttagcc
     3481 agggtaaatg ccatatcccc cgtacagatt gcttcatttg gaggtacatt tctgtgatca
     3541 gatttgtccg gtaggccgtg gtttagatgg aaatggcaac attcccatta ccgtcggtga
     3601 agcctcacct tcgtataccg ctgaccaaca gccccgcagt gccaggtacc aagacgcctt
     3661 ttcaatatcg attcatctga atccggcgta agccagacat gaagctggac cgcggcgctt
     3721 aatactggtc ggtagttact atcgattaca agtggctcat tggcgacgaa gactgtttta
     3781 gtcaaataga tcgcacaaat tatcacgatc ccgagcccag acgggcttat tgtcactcac
     3841 taatgcaggc gaattgtttt tagctacgct cgaacgacag cgatcatctg aagtgggcga
     3901 tcgctctaga gctagcgaat tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3961 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4021 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4081 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgct
//
