LOCUS       sequence-191892-        4031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the straight four-helix-bundle shown in figure 4A of 
            associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_4hb_straight
FEATURES             Location/Qualifiers
     source          1..4031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..38
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(155..172)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(326..914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(406..425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1216..2010
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1270..1289)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1880..1899
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2175..2193
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2231..2253)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2353..2372
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3968..3990)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3979..3995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4003..4009,4010..4027,4028..4031,1..2))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
       61 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      121 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      481 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      541 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      601 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      661 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      721 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      781 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      841 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      901 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      961 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1021 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa aaatattccg
     1081 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     1141 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     1201 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     1261 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     1321 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     1381 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     1441 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     1501 gaagtgccgg ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc
     1561 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     1621 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     1681 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     1741 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     1801 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     1861 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     1921 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     1981 ttctatcgcc ttcttgacga gttcttctga accggtaata ttattgaagc atttatcagg
     2041 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2101 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2161 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     2221 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2281 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     2341 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccaattg gtcgacctcg
     2401 agttaattaa cgtatattgg atgaaatact agctaatact gtggaaaggc tagaggtctg
     2461 ctcgacgtaa ctgacgtgag aactaacgat agcatcggtt accagtacat gtataactaa
     2521 acctcgctgg acatagatag tccaagagcg ctgattacat tggaaaaaga gcaactcgta
     2581 tgttcgagat ctatgaatta tgagacgaaa acgcctccac ggactataaa tgtcggttgc
     2641 cgtctgccgg atcctcagtc agtaaattgg actggactat ccgagcgttg tccatgcctt
     2701 tatgcttgcg ttagaggacc tatcacaagt acaagtctta ctgtatatcc ggactaacac
     2761 cggctctgag gttgacgcca cagagggtac cgcagccctc cttagcatag ctagccctaa
     2821 gtgagcttca ggccatgaga cagcctcatc agcggatcat ctcgatggat gagcgtcagg
     2881 gggtagtagg tattagaggt gcgctgtagg tatcgactgt agattcgtat tcatgttata
     2941 acggacgggt atcctccgat ataatgcccg gactctatcc aggatggaag aaattcgggc
     3001 cccagtggct cattcgtgaa ccgacaactc ttgacacccg ataggacgcc cgttcggtaa
     3061 atgtggattc accggaaccg tgtatgtgga cggtgatcga gacaaaaacg gtgtatactt
     3121 gcatagaact ccacaagaaa ttgccacggt ggcggcaatc ggtcgttgta ccgggaattc
     3181 ggcgcccacg cctaagccga catgggtcgg cgaggcactc ccggccagca ccgccggaga
     3241 ctttacagcc tgactttttg aagtccattt aggaccgtaa gattttatca ctgtaaacat
     3301 gttttcacca ggctaccatc cggtccgtgt tagcgatgca caatgtgggg tacgatacag
     3361 actagaatgc tcgcgtattg aatggcggga tgttgatacc agttcagtag taccgatagt
     3421 atcgtttcaa acatatactg catgttcgag atcaatcgat agtaaccttt gggcgtgtac
     3481 gaggctttcg acgaaacgtt acgcctcgga ctgatcccaa cgcgaccgca aaccctgtga
     3541 aacgtcctgc cctattccac gacaactaaa gctaagagtt caagtgcatc tggtctgccg
     3601 caattagcct aatttaattc tgaaacttag ccagggtaaa tgccatatcc cccgtacaga
     3661 ttgcttcatt tggaggtaca tttctgtgat cagatttgtc cggtaggtca atatcgattc
     3721 atctgaatca acattcccat taccgtcggg ctggaccgcg gcgcttaata cgctgaccaa
     3781 cagccccgca gttacaagtg gctcattggc gatgttcaat atgagagggc aatagatcgc
     3841 acaaattatc acgaagaaca ttggtggcat acattttgtc actcactaat gcaggcaagc
     3901 tgcatatgtg atgacttgaa cgacagcgat catctgaagt gggcgatcgc tctagagcta
     3961 gcgaattcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4021 cataaagtgt a
//
