LOCUS       sequence-191914-        2912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PMDH3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pMDH3
FEATURES             Location/Qualifiers
     source          1..2912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1208..1224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1208..1224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1272..1303
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1304..1406
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1407..2222
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1480..1499)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2246..2273
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2285..2872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2365..2384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2912,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggagtag cttgggtagg acttgacaag tacggcttcc
      181 gtggtcatac caaacgcctt tgttaccgtt ggctatacct aatgaccaag gcatttgtgg
      241 attataacgg tatcgtagtt gaaaaatatg acgtaaccac tggtactagc ccccacaagg
      301 ttgatgctga atacgggaat caaggtgccg attttaaagg agtagccact gaagggtttg
      361 gctgggtcaa tgcctctttt attttgggat taacctactt agatgtccaa ggcatccgtg
      421 cgataggcgc cgttacgtcc cctgatgtat ttttcaggaa gctcaaacct tgggaacgcg
      481 caagttatgg cctaaggcca tgtaacgaga tagtcaagtc aaactagaag tatacggttt
      541 ccccgcagaa atagcagaaa taggcgacaa atacatacaa cattttcatt gtgatagggg
      601 gcggcggttc ctaggaggga caacccccag aaaccttgta gactacgttt tcacgacgat
      661 gggttattac tgtaaaggaa gaatatacta cccaccagtt gaatgtttga acggatcaaa
      721 ggtcgaaggg agtacacggc ccaaccaacg tagctaccgg agaaagcaag actttcccaa
      781 accaaatagc tccgggtttc ttctccggca acccgtcagt ttttgtgtgg ccggacaaaa
      841 attcgcaccc tcagtctaat tgaaaggtcg ggctccgagc tctaggcgtt tgcgcatgta
      901 atattgcatc ccctcccata gataatactg cgcgaacaca gggtgcaaat tatgatgacc
      961 acacatgcca gtgaccaaaa cagtttttta gtctttaaaa accctcggaa cttctgagta
     1021 tataaaggct tctcatttcc tacaagcaaa caaagaagaa acttccactt tctaactttt
     1081 tatctataga ctttagagtt acaaccaacg aacaataaca acatggcgtc ttcgaattcg
     1141 cgcgtgatct tacggcatta tacgtatgat cggtccacga tcagctagat tatctagtca
     1201 gcttgatgtc atagctgttt cctgaggctc aatactgacc atttaaatca tacctgacct
     1261 ccatagcaga aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag
     1321 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     1381 gattttcagg agctaaggaa gctaaaatga gccatattca acgggaaacg tcttgcttga
     1441 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1501 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     1561 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcaggc
     1621 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1681 atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag
     1741 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1801 attcgattcc tgtttgtaat tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg
     1861 cgcaatcacg aatgaataac ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg
     1921 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact cttgccattc tcaccggatt
     1981 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2041 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2101 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2161 gtattgataa tcctgatatg aataaattgc agtttcactt gatgctcgat gagtttttct
     2221 aatgaggacc taaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg
     2281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga
     2341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2701 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2761 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2881 tttgattttc taccgaagaa aggcccaccc gt
//
