LOCUS       sequence-191915-        2297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector, no ccDB gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4 no ccDB (686-1)
FEATURES             Location/Qualifiers
     source          1..2297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    77..176
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(245..344)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(363..386)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(394..421)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(553..596)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(764..1352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(844..863)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1442..2251)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2165..2184
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2243..2262
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag
       61 agctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      121 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtctag
      181 atatctcgag tgcggccgcg aattcggtac cggatccagt cgactgaatt ggttcccatg
      241 gtggagcctg cttttttgta caaagttggc attataaaaa agcattgctt atcaatttgt
      301 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttggggccc gagcttaagt
      361 aactaactaa caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      421 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
      481 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
      541 gacaaacaac agataaaacg aaaggctcag tcggaagact gggcctttcg ttttatttga
      601 tgcctggcag ttccctactc tcgcttagta gttagacgtc cccgagatcc atgctagcgg
      661 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      721 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      781 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      841 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      901 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      961 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1021 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1081 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1141 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1201 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1261 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1381 ctgacgctca gtggaacggg gcccaatctg aataatgtta caaccaatta accaattctg
     1441 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     1501 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     1561 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     1621 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     1681 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     1741 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     1801 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     1861 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     1921 attcttctaa tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat
     1981 caccaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     2041 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     2101 acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga
     2161 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     2221 gcctcgacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     2281 cagacagttt tattgtt
//
